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008			100301s1998 xxu o |||| 0|eng d
020			_a9781592592548
024	7		_a10.1385/0896034720 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aRB170 _b1998 EB
245	0	0	_aFree Radical and Antioxidant Protocols _cedited by Donald Armstrong
250			_a1st edition 1998
264		1	_aTotowa, NJ _bHumana Press _c1998
300			_a1 recurso en línea (XVII, 455 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v108
505	0		_aTechniques for the Measurement of Oxidative Stress -- Oxygen Consumption Methods -- Spin Trapping and Electron Paramagnetic Resonance Spectroscopy -- In Vivo Detection of Free Radicals in Real Time by Low-Frequency Electron Paramagnetic Resonance Spectroscopy -- Single Photon Counting -- Measurement of Reactive Oxygen Species in Whole Blood and Mononuclear Cells Using Chemiluminescence -- Assay of Phospholipid Hydroperoxides by Chemiluminescence-Based High-Performance Liquid Chromatography -- Sensitive and Specific Fluorescent Probing of Oxidative Stress in Different Classes of Membrane Phospholipids in Live Cells Using Metabolically Integrated cis-Parinaric Acid -- Aromatic Hydroxylation -- Simple Assay for the Level of Total Lipid Peroxides in Serum or Plasma -- Simple Procedure for Specific Assay of Lipid Hydroperoxides in Serum or Plasma -- Antibodies Against Malondialdehyde-Modified Proteins -- Oxidized LDL and Lp(a) -- Oxidized and Unoxidized Fatty Acyl Esters -- Synthesis of Lipid and Cholesterol Hydroperoxide Standards -- Separation of Hydroxy and Hydroperoxy Polyunsaturated Fatty Acids By High-Pressure Liquid Chromatography -- Products of Creatinine with Hydroxyl Radical as a Useful Marker of Oxidative Stress In Vivo -- Quantitative Analysis of Peptide and Protein Changes in Ischemic Hippocampal Tissue by HPLC -- Electrochemical Detection of 8-Hydroxy-2-Deoxyguanosine Levels in Cellular DNA -- Nitric Oxide Synthase -- Quantitation of Heme Oxygenase (HO-1) Copies in Human Tissues by Competitive RT/PCR -- A Fluorescent-Based Assay for Measurement of Phospholipase A2 Activity -- Autofluorescent Ceroid/Lipofuscin -- In Vivo Technique for Autofluorescent Lipopigments -- An Ex Vivo Erythrocyte Model for Investigating Oxidative Metabolism -- Purification of Vesicular Carriers from Rat Hepatocytes by Magnetic Immunoadsorbtion -- Techniques for the Measurement of Antioxidant Activity -- Simultaneous Determination of Serum Retinol, Tocopherols, and Carotenoids by HPLC -- EPR Measurements of Nitric Oxide-Induced Chromanoxyl Radicals of Vitamin E -- Nonvitamin Plasma Antioxidants -- Regulatory Antioxidant Enzymes -- In Vitro Screening for Antioxidant Activity -- Antioxidant Activity of Low-Density Lipoprotein -- Lipoic Acid as an Antioxidant -- Reduced Glutathione and Glutathione Disulfide -- Analysis of Coenzyme Q10 Content in Human Plasma and Other Biological Samples -- Techniques Applicable to Both Oxidative Stress and Antioxidant Activity -- A Simple Luminescence Method for Detecting Lipid Peroxidation and Antioxidant Activity In Vitro -- Analysis of Oxidation and Antioxidants Using Microtiter Plates -- Trace Element Analysis of Biological Samples by Analytical Atomic Spectroscopy -- Energy Dispersive X-Ray Microanalysis -- Pro-Oxidant and Antioxidant Effects of Estrogens -- Artificial Radical Generating and Scavenging Systems.
520			_aIn Free Radical and Antioxidant Protocols, seventy-six leading international authorities describe cutting-edge methodologies for quantifying free radical and antioxidant analytes in tissue and body fluids using experimental models and in vitro procedures. These user-friendly and easily reproducible techniques cover the essential tasks, including radical generating systems, direct measurement or trapping of reactive radical species and acute-phase proteins, and measurement of metabolic intermediates derived from the oxidation of lipids, proteins, and nucleic acids. There are also methods for the determination of vitamin, enzymatic, and water-soluble antioxidants, as well as of essential micronutrients and cofactors. The techniques take advantage of new instrumentation-probes, photon counting, chemiluminescence, and caged compounds, with an emphasis on HPLC-and are adaptable to a wide range of applications. Free Radical and Antioxidant Protocols provides state-of-the-art methodology and biotechnology in a convenient format for both academic and corporate biomedical scientists. The detailed, laboratory-tested free-radical assays, many of them presented here for the first time, will illuminate the study of both primary and secondary oxidative stress and contribute significantly to our understanding of the many disorders associated with this process.
988			_aSpringer_Protocols_1998
650		7	_2embne _9153725 _aAntioxidantes
650		7	_2embne _9148373 _aRadicales libres (Química)
776	0	8	_iPrinted edition: _z9781617370496
776	0	8	_iPrinted edition: _z9780896034723
776	0	8	_iPrinted edition: _z9781489942487
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896034720 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
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998			_b12/2023 _dz _eb _zSI