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008			100301s2001 xxua o |||| 0|eng d
020			_a9781592592319
024	7		_a10.1385/1592592317 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP88.15 _b2001 EB
245	0	0	_aAdipose Tissue Protocols _cedited by Gérard Ailhaud
250			_a1st edition 2001
264		1	_aTotowa, NJ _bHumana Press _c2001
300			_a1 recurso en línea (XIII, 334 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v155
505	0		_aChoosing an Adipose Tissue Depot for Sampling -- Morphologic Techniques for the Study of Brown Adipose Tissue and White Adipose Tissue -- Confocal Microscopy of Adipocytes -- Cellularity Measurements -- Subcellular Fractionation of Adipocytes and 3T3-L1 Cells -- Quantification of Lipid-Related mRNAs by Reverse Transcription-Competitive Polymerase Chain Reaction in Human White Adipose Tissue Biopsies -- Glycogen Synthase Activity in Adipose Tissue -- Assays of Lipolytic Enzymes -- Assays of Lipogenic Enzymes -- Assays of Adrenergic Receptors -- Transfection of Adipocytes and Preparation of Nuclear Extracts -- Assay of Membrane Transport of Long-Chain Fatty Acids by Adipocytes -- Assays of Glucose Entry, Glucose Transporter Amount, and Translocation -- Methods to Study Phosphorylation and Activation of the Hormone-Sensitive Adipocyte Phosphodiesterase Type 3B in Rat Adipocytes -- Measurements of Glucose Conversion to its Metabolites -- Metabolite and Ion Fluxes and Ion Channels in Brown and White Adipocytes -- Culture of Adipose Tissue and Isolated Adipocytes -- Cultures of Adipose Precursor Cells from Brown Adipose Tissue and of Clonal Brown-Adipocyte-Like Cell Lines -- Cultures of Adipose Precursor Cells and Cells of Clonal Lines from Animal White Adipose Tissue -- Cultures of Human Adipose Precursor Cells -- Measurements of Peptide and Nonpeptide Secretory Products from Adipocytes -- Assessment of White Adipose Tissue Metabolism by Measurement of Arteriovenous Differences -- Measurement of Adipose Tissue Blood Flow -- Respiratory and Thermogenic Capacities of Cells and Mitochondria from Brown and White Adipose Tissue -- Measurements of White Adipose Tissue Metabolism by Microdialysis Technique -- Immunoassay Measurements of Leptin.
520			_aAdipose tissue is now recognized as a widely dispersed secretory organ that exhibits autocrine, paracrine, and endocrine properties, and plays a significant role in obesity, the most common health problem in industrialized countries. In Adipose Tissue Protocols, Gérard Ailhaud and a team of laboratory experts and clinicians describe in step-by-step detail the major techniques needed for the study of adipose tissue and cells. Drawn from both in vivo and in vitro studies, these readily reproducible methods cover a broad range of techniques, including the choice of adipose tissue depot and of morphological techniques for work on both brown adipose tissue (BAT) and white adipose tissue (WAT). Major treatment is accorded the isolation, subcellular fractionation, and transfection of low density adipocytes, as well as the metabolic aspects of nutrient uptake and key assays of nutrient and ion fluxes. Also covered are: biopsies and quantification of lipid-related mRNAs; cultures of adipose precursor cells from WAT and BAT; measurements of adipose secretory products; assessment of WAT metabolism in vivo; and assays of lipid-related enzymes. Innovative and highly practical, Adipose Tissue Protocols offers endocrinologists, physiologists, cell biologists, and pharmacologists a gold-standard collection of proven methods for effective nutritional, physiological, and molecular-level research on adipose tissue.
988			_aSpringer_Protocols_2001
650		7	_2embne _9667182 _aTejido adiposo
776	0	8	_iPrinted edition: _z9781489942395
776	0	8	_iPrinted edition: _z9781489942388
776	0	8	_iPrinted edition: _z9780896037472
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592592317 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _eb _zSI