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020 _a9781627033336
024 7 _a10.1007/978-1-62703-333-6
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQK725
_b2013 EB
245 0 0 _aPlant Meiosis :
_bMethods and Protocols
_cedited by Wojciech P. Pawlowski, Mathilde Grelon, Susan Armstrong
250 _a1st edition 2013
264 1 _aTotowa, NJ
_bHumana Press
_c2013
300 _a1 recurso en línea (XV, 238 páginas)
_b52 ilustraciones, 28 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
505 0 _aSpreading and Fluorescence in situ Hybridization (FISH) of Male and Female Meiocyte Chromosomes from Arabidopsis thaliana for Cytogenenetical Analysis -- Analysis of Plant Meiotic Chromosomes by Chromosome Painting -- Using Sequential Fluorescence and Genomic in situ Hybridization (FISH and GISH) to Distinguish the A and C Genomes in Brassica napus -- Labeling Meiotic Chromosomes in Maize with Fluorescence in situ Hybridization -- Examining Female Meiocytes of Maize by Confocal Microscopy -- Three-dimensional Acrylamide Fluorescence in situ Hybridization (FISH) for Plant Cells -- Analyzing Maize Meiotic Chromosomes with Super-resolution Structured Illumination Microscopy -- Live Imaging of Chromosome Dynamics -- Immunolocalization of Meiotic Proteins in Brassicacae - Method -- Immunolocalization of Meiotic Proteins in Arabidopsis thaliana - Method 2 -- Immunolocalization Protocols for Visualizing Meiotic Proteins in Arabidopsis thaliana - Method 3 -- A Time Course for the Analysis of Meiotic Progression in Arabidopsis thaliana -- Analyzing Meiotic Chromosomes in Rice -- Analysing Meiosis in Barley -- Preparing SC Spreads with RNs for EM Analysis -- Analysis of the Synaptonemal Complex in Brassica using TEM -- Preparing Thin Sections of Meiotic Nuclei for Transmission Electron Microscopy -- Characterization of Meiotic Non-crossover Molecules from Arabidopsis thaliana Pollen -- Chromatin Immunoprecipitation for Studying Chromosomal Localization of Meiotic Proteins in Maize -- Analyzing the Meiotic Transcriptome using Isolated Meiocytes of Arabidopsis thaliana -- Analysis of Meiotic Protein Complexes from Arabidopsis and Brassica using Affinity-based Proteomics -- Identifying Meiotic Mutants in Arabidopsis thaliana.
520 _aMeiosis is one of the most critical processes in eukaryotes, required for continuation of species and generation of new variation.  In plants, meiotic recombination is by far the most important source of genetic variation.  In Plant Meiosis: Methods and Protocols, expert researchers in the field detail methods for molecular cytogenetics and chromosome analysis in plants.  These state-of -the-art protocols allow studying the organization and behavior of the genetic material in a wide range of both model and crop species.  Written in the highly successful Methods in Molecular Biology™series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.    Authoritative and practical, Plant Meiosis: Methods and Protocols provides and extensive list of protocols developed and used in a number of laboratories at the cutting edge of meiosis and chromosome research.
988 _aSpringer_Protocols_2013
650 7 _2embne
_9146302
_aCélulas vegetales
_vManuales de laboratorio
776 0 8 _iPrinted edition:
_z9781627033329
776 0 8 _iPrinted edition:
_z9781627033343
776 0 8 _iPrinted edition:
_z9781493959723
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-333-6
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b12/2023
_dz
_eb
_zSI