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007			cr nn 008mamaa
008			121029s2013 xxua o |||| 0|eng d
020			_a9781603273534
024	7		_a10.1007/978-1-60327-353-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR67 _b2013 EB
245	0	0	_aPCR Detection of Microbial Pathogens _cedited by Mark Wilks
250			_a2nd edition 2013
264		1	_aTotowa, NJ _bHumana Press _c2013
300			_a1 recurso en línea (XI, 317 páginas) _b30 ilustraciones, 19 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v943
505	0		_aReal-time Quantitative PCR, Pathogen Detection and MIQE -- Overcoming Inhibition in Real-time Diagnostic PCR -- Quality in the Molecular Microbiology Laboratory -- Pre-Analytical Sample Treatment and DNA Extraction Protocols for the Detection of Bacterial Pathogens from Whole Blood -- Detection of Bacterial Contamination in Platelet Concentrates using Flow Cytometry and Real-time PCR Methods -- Multiplex Real-time PCR Assay for the Detection of Meticillin-Resistant Staphylococcus aureus and Panton-Valentine Leukocidin from Clinical Samples -- PCR Detection of Haemophilus influenzae from Respiratory Specimens.-  Rapid Detection of Atypical Respiratory Bacterial Pathogens by Real-time PCR -- Use of Multiple Real-time PCR Assays for the Diagnosis of Bodetella spp in Clinical Specimens -- Detection of Mycoplasma pneumoniae by Real-time PCR -- Real-Time PCR Assay for the Diagnosis of Pneumocystis jirovecii Pneumonia -- Rapid Identification of Mycobacteria and Rapid Detection of Drug Resistance in Mycobacterium tuberculosis in Cultured Isolates and in Respiratory Specimens -- Direct Detection of Mycobaterium ulcerans in Clinical Specimens and Environmental Samples -- Detection of Bartonella spp. DNA in Clinical Specimens using an Internally Controlled Real-time PCR Assay -- Direct Identification of Multiple Genital Pathogens in Urine using Multiplex PCR-based Reverse Line Blot Assays -- Diagnosis of Clostridium difficile Infection using Real-time PCR -- Detection of Pathogenic Leptospira spp. through Real-time PCR (qPCR) Targeting the LipL32 Gene -- Sensitive and Rapid Detection of Campylobacter jejuni and Campylobacter coli using Loop-mediated Isothermal Amplification -- PCR Detection of Helicobacter pylori in Clinical Samples -- Rapid Detection of the Escherichia coli Genospecies in Water by Conventional and Real-time PCR -- Multiplex Real Time PCR (MRT-PCR) for Diarrheagenic Escherichia coli.
520			_aPCR methods for the detection of microbial pathogens have made relatively little impact in diagnostic microbiology laboratories due to the common decision to use expensive commercially produced tests rather than the cheaper alternative of developing one's own tests or introducing tests developed by other workers. PCR Detection of Microbial Pathogens, Second Edition presents alternatives to commercially produced PCR methods to detect microbial pathogens. Although most of the chapters in this book are devoted to the detection of specific pathogens, the first  chapters in this book should appeal to anyone working in this field regardless of their particular interests. Although PCR tests can often be made to work with relatively little effort, it is often unclear how efficient the PCR test is, how inhibitory the specimen containing the pathogen of interest is and how the test can be quality controlled. All of which are of great importance in developing tests for diagnostic use. These topics are covered  in great depth at the beginning of the book. The main part of the book is devoted to describing methods for the detection of a wide range of pathogens and from widely different specimens and situations. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and accessible, PCR Detection of Microbial Pathogens, Second Edition serves microbiologists regardless of their particular interest because, when used together with the general principles, the sheer variety of procedures provided here enables the reader to design and introduce diagnostic tests in the laboratory with confidence.
988			_aSpringer_Protocols_2013
650		7	_2embne _9413457 _aDiagnóstico microbiológico
776	0	8	_iPrinted edition: _z9781607616375
776	0	8	_iPrinted edition: _z9781603273527
776	0	8	_iPrinted edition: _z9781493960996
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60327-353-4 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _eb _zSI