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008			131104s2014 xxua f |||| 0|eng d
020			_a9781461487944
024	7		_a10.1007/978-1-4614-8794-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aRC337 _b2014 EB
245	0	0	_aCurrent Laboratory Methods in Neuroscience Research _cedited by Huangui Xiong, Howard E. Gendelman
250			_a1st edition 2014
264		1	_aNew York, NY _bSpringer International Publishing _c2014
300			_a1 recurso en línea (XIII, 585 páginas) _b189 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aSpringer Protocols Handbooks _x1949-2456
505	0		_aSection 1: Neurohistological techniques -- 1. Brain tissue preparation, sectioning and staining -- 2. Brain stereotactic injections -- 3. Tract tracing at light and electron microscopy -- 4. Stereological analysis of neurological tissues -- Section 2: In vitro preparations -- 5. Preparation and use of rodent hippocampal brain slices -- 6.  Single-Cell Neuronal Dissociation For Electrophysiological Studies -- 7. Isolation and Culture of Human Neurons, Microglia and Astrocytes -- 8. Isolation and Culture of Neural Stem/progenitor Cells -- 9. Isolation of mitochondria from brain tissue and cells -- 10. Isolation of synaptosomes from archived brain tissues -- 11. Xenopus oocytes isolation and microinjection -- Section 3: Leukocyte isolation and application in neuroscience -- 12. Centrifugal elutriation for studies of neuroimmunity -- 13. Flow cytometry in neuroscience research -- Section 4: Standard Laboratory Nucleic Acid and Protein Detections -- 14. Western Blotting Technique in Biomedical Research -- 15. Polymerase chain reaction (PCR) and Real-time RT PCR -- Section 5: Nanomedicine -- 16. Nanoformulations -- 17. Neuronanomedicine -- Section 6: Bioimaging -- 18. Confocal imaging of nerve cells -- 19. Magnetic resonance imaging (MRI) -- 20. X-Ray, Positron Emission, and Single Photon Emission Tomographic Bioimaging -- 21. Non-Invasive Neurophysiological Imaging with Magnetoencephalography -- 22. Multiphoton Brain Imaging -- Section 7: Neuroelectrophysiology -- 23. Techniques for Extracellular Recordings -- 24. "Blind" patch-clamp recordings from rodent hippocampal slices -- 25. Whole-cell patch clamp recordings -- Section 8: Immunohistochemistry and autoradiography -- 26. Analysis of Receptor Binding And Quantitative Autoradiography -- 27. Techniques in Immunohistochemistry and immunocytochemistry -- Section 9: Analysis of Gene Expression -- 28. Protein identification by mass spectrometry: Proteomics -- 29. DNA Microarrays as a Tool for Neurosciences Research -- 30. Metabolomics -- 31. Bioinformatic Methods and Resources for Neuroscience Research -- Section 10: Animal Models: Behavior and Pathology -- 32. Motor function in rodent models of neurodegenerative disorders -- 33. Humanized mice -- 34. Animal models for PD and ALS -- 35. Animal models for Alzheimer's Disease -- 36. Animal Models: Behavior and Pathology.
520			_aCurrent Laboratory Methods in Neuroscience Research is a research manual for both students and seasoned researchers. It focuses on commonly-used techniques employed in neuroscience research, presented in a simple, step-by-step manner for laboratory use. The manual also offers a "blueprint" for  bench-to-bedside research designed to facilitate multidisciplinary neuroscience pursuits. Sections include coverage of neurohistological techniques, in vitro preparations, leukocyte isolation and application in neuroscience, standard laboratory nucleic acid and protein detections, nanomedicine, bioimaging, neuroelectrophysiology, immunohistochemistry and autoradiography, analysis of gene expression, and animal models.
988			_aSpringer_Protocols_2014
650		7	_2embne _9139059 _aPsicoterapia _xInvestigación
650		7	_2embne _9158907 _aNeurociencias
776	0	8	_iPrinted edition: _z9781461487951
776	0	8	_iPrinted edition: _z9781461487937
776	0	8	_iPrinted edition: _z9781493952168
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4614-8794-4 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _eb _zSI