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008			160802s2016 xxua o |||| 0|eng d
020			_a9781493936373
024	7		_a10.1007/978-1-4939-3637-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP552 .M44 _b2016 EB
245	0	0	_aHeterologous Expression of Membrane Proteins : _bMethods and Protocols _cedited by Isabelle Mus-Veteau
250			_a2nd edition 2016
264		1	_aNew York, NY _bSpringer International Publishing _c2016
300			_a1 recurso en línea (XII, 322 páginas) _b66 ilustraciones, 39 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1432
505	0		_aCell-Free Production of Membrane Proteins in Escherichia coli Lysates for Functional and Structural Studies -- Membrane Protein Production in the Yeast, S. cerevisiae -- Membrane Proteins Production in Escherichia coli: Protocols and Rules -- Codon Optimizing for Increased Membrane Protein Production: A Minimalist Approach -- Generation of Tetracycline-Inducible Mammalian Cell Lines by Flow Cytometry for Improved Overproduction of Membrane Proteins -- Membrane Protein Production in Lactococcus lactis for Functional Studies -- Expression of Viral Envelope Glycoproteins in Drosophila melanogaster S2 Cells -- Leishmania tarentolæ as a Promising Tool for Expressing Polytopic and Multi-Transmembrane Spans Eukaryotic Membrane Proteins: The Case of the ABC Pump ABCG6 -- Overexpression, Membrane Preparation, and Purification of a Typical Multidrug ABC Transporter BmrA -- Expression of Eukaryotic Membrane Proteins in Pichia pastoris -- Integral Membrane Protein Expression in Saccharomyces cerevisiae -- High-Throughput Baculovirus Expression System for Membrane Protein Production -- Small-Scale Screening to Large-Scale Over-Expression of Human Membrane Proteins for Structural Studies -- Purification of Human and Mammalian Membrane Proteins Expressed in Xenopus laevis Frog Oocytes for Structural Studies -- Membrane Protein Solubilization and Composition of Protein Detergent Complexes -- Detergent-Free Membrane Protein Purification -- Conformational Dynamics and Interactions of Membrane Proteins by Hydrogen/Deuterium Mass Spectrometry -- Lessons from an a-Helical Membrane Enzyme: Expression, Purification, and Detergent Optimization for Biophysical and Structural Characterization -- Method to Screen Multidrug Transport Inhibitors Using Yeast Over-Expressing a Human MDR Transporter.
520			_aThis detailed volume encompasses chapters from leading experts in the area of membrane proteins who describe step-by-step protocols developed these last few years to improve the functional production and stabilization of recombinant integral membrane proteins (IMPs). Membrane proteins play a key role in numerous pathologies such as cancer, cystic fibrosis, epilepsy, hyperinsulinism, and Alzheimer's disease, yet studies on these and other disorders are hampered by a lack of information about the proteins involved. This book sets out to aid researchers in rectifying this situation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Heterologous Expression of Membrane Proteins: Methods and Protocols, Second Edition serves as an ideal guide for scientists attempting to delve deeper into the myriad unique IMP structures.
988			_aSpringer_Protocols_2016
650		7	_2embne _9671893 _aProteínas de membranas
776	0	8	_iPrinted edition: _z9781493936359
776	0	8	_iPrinted edition: _z9781493936366
776	0	8	_iPrinted edition: _z9781493981021
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-3637-3 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _eb _zSI