000			05183nam a2200397 i 4500
999			_c393011 _d393011
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003			ES-MaUEC
005			20231215123207.0
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007			cr nn 008mamaa
008			100316s2009 xxua o |||| 0|eng d
020			_a9781597455213
024	7		_a10.1007/978-1-59745-521-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH603 .M5 _b2009 EB
245	0	0	_aMitochondrial DNA : _bMethods and Protocols _cedited by Jeffrey A Stuart
250			_a2nd edition 2009
264		1	_aTotowa, NJ _bHumana Press _c2009
300			_a1 recurso en línea (XII, 404 páginas) _b74 ilustraciones, 1 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v554
505	0		_aMitochondrial DNA Replication, Transcription, Translation, and Respiratory Complex Assembly -- Biochemical Isolation of mtDNA Nucleoids from Animal Cells -- Analysis of Mitochondrial DNA by Two-Dimensional Agarose Gel Electrophoresis -- Comparative Purification Strategies for Drosophila and Human Mitochondrial DNA Replication Proteins: DNA Polymerase ? and Mitochondrial Single-Stranded DNA-Binding Protein -- Functional Analysis of Mutant Mitochondrial DNA Polymerase Proteins Involved in Human Disease -- Preparation of Human Mitochondrial Single-Stranded DNA-Binding Protein -- Methods for Assessing Binding of Mitochondrial Transcription Factor A (TFAM) to DNA -- Inducible Expression in Human Cells, Purification, and In Vitro Assays for the Mitochondrial DNA Helicase Twinkle -- Purification Strategy for Recombinant Formsof the Human Mitochondrial DNA Helicase -- Methods for Studying Mitochondrial Transcription Termination with Isolated Components -- Oxidative Phosphorylation: Synthesis of Mitochondrially Encoded Proteins and Assembly of Individual Structural Subunits into Functional Holoenzyme Complexes -- Mitochondrial DNA Damage and Repair -- Reactive Oxygen Species Production by Mitochondria -- Measuring mtDNA Damage Using a Supercoiling-Sensitive qPCR Approach -- Quantitative Analysis of Oxidized Guanine, 8-Oxoguanine, in Mitochondrial DNA by Immunofluorescence Method -- In Vitro Measurement of DNA Base Excision Repair in Isolated Mitochondria -- Targeting Repair Proteins to the Mitochondria of Mammalian Cells Through Stable Transfection, Transient Transfection, Viral Transduction, and TAT-Mediated Protein Transduction -- Construction and Characterization of a Cell Line Deficient in Repair of Mitochondrial, but Not Nuclear, Oxidative DNA Damage -- Mitochondrial DNA Mutations -- Mitochondrial DNA Oxidative Damage and Mutagenesis in Saccharomyces cerevisiae -- Determination of DNA Mutation Load in Human Tissues Using Denaturing HPLC-Based Heteroduplex Analysis -- Rapid Identification of Unknown Heteroplasmic Mitochondrial DNA Mutations with Mismatch-Specific Surveyor Nuclease -- Collection of Isolated Cells for Studying Mitochondrial DNA Mutations Within Individual Cells -- Quantitative Analysis of Somatic Mitochondrial DNA Mutations by Single-Cell Single-Molecule PCR -- Measuring DNA Precursor Pools in Mitochondria -- Establishment of Human Cell Lines Lacking Mitochondrial DNA.
520			_aSince the publication of the first edition, the number of unique heritable mtDNA mutations recognized as being associated with bioenergetic dysfunction, cell death and disease has grown. Likewise, our understanding of the basic biology of somatic mtDNA mutations continues to improve. In Mitochondrial DNA: Methods and Protocols, Second Edition, specialists from eight countries share their expertise, providing detailed protocols for studying many aspects of mtDNA. The volume is divided into three sections, which cover the transduction of information from mtDNA to functionally active respiratory complexes, mitochondrial reactive oxygen species (ROS) production as well as mtDNA damage and its repair, and the identification and quantification of heteroplasmic mtDNA mutations. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Mitrochondrial DNA: Methods and Protocols, Second Edition promises to aid researchers in further expanding our knowledge of this vital area of cell biology.
988			_aSpringer_Protocols_2009
650		7	_2embne _9147980 _aMitocondrias
776	0	8	_iPrinted edition: _z9781607610373
776	0	8	_iPrinted edition: _z9781934115602
776	0	8	_iPrinted edition: _z9781617794957
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-521-3 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _eb _zSI