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008			111201s2012 xxu| fo |||| 0|eng d
020			_a9781617793431
024	7		_a10.1007/978-1-61779-343-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQM571 _b2012 EB
245	0	0	_aMyogenesis _bMethods and Protocols _cedited by Joseph X. DiMario
250			_a1st edition 2012
264		1	_aTotowa, NJ _bHumana Press _c2012
300			_a1 recurso en línea (XV, 558 páginas) _b86 ilustraciones, 15 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v798
505	0		_aIsolation and Characterization of Human Fetal Myoblasts -- Skeletal Muscle Satellite Cells: Background and Methods for Isolation and Analysis in a Primary Culture System -- Isolation of Muscle Stem Cells by Fluorescence Activated Cell Sorting (FACS) Cytometry -- Mouse and Human Mesoangioblasts: Isolation and Characterization from Adult Skeletal Muscles -- Direct Electrical Stimulation of Myogenic Cultures for Analysis of Muscle Fiber Type Control -- Single Muscle-Fiber Isolation and Culture for Cellular, Molecular, Pharmacological, and Evolutionary Studies -- Somite Unit Chronometry to Analyze Teratogen Phase Specificity in the Paraxial Mesoderm -- Analysis of Skeletal Muscle Development in Drosophila -- Immunocytochemistry to Study Myogenesis in Zebrafish -- Immunofluorescent Localization of Proteins in C. elegans Muscle -- Resistance Loading and Signaling Assays for Oxidative Stress in Rodent Skeletal Muscle -- Analysis of Skeletal Muscle Hypertrophy in Models of Increased Loading -- Protein Overexpression in Skeletal Muscle Using Plasmid-Based Gene Transfer to Elucidate Mechanisms Controlling Fiber Size -- In Vivo Measurement of Muscle Protein Synthesis Rate Using the Flooding Dose Technique -- Recombinant Adeno-Associated Viral Vector Production and Purification -- Generation of Lentiviral Vectors for Use in Skeletal Muscle Research -- Generating Tamoxifen-Inducible Cre Alleles to Investigate Myogenesis in Mice -- Gene Profiling Studies in Skeletal Muscle by Quantitative Real-Time Polymerase Chain Reaction Assay -- Analysis of Lipid Profiles in Skeletal Muscles -- Proteomic Analysis of Dystrophic Muscle -- Detection of Calcium Release via Ryanodine Receptors -- Measurement of Calcium Release Due to Inositol Trisphosphate Receptors in Skeletal Muscle -- Detection of Calcium Sparks in Intact and Permeabilized Skeletal Muscle Fibers -- Analysis of Calcium Transients in Cardiac Myocytes and Assessment of the Sarcoplasmic Reticulum Ca2+-ATPase Contribution -- Analysis of Muscle Gene Transcription in Cultured Skeletal Muscle Cells -- Analysis of Fiber Type Differences in Reporter Gene Expression of b-Gal Transgenic Muscle -- Determination of Gene Promoter Activity in Skeletal Muscles In Vivo -- Determination of MiRNA Targets in Skeletal Muscle Cells -- shRNA-Mediated Gene Knockdown in Skeletal Muscle -- Detection of NF-kB Activity in Skeletal Muscle Cells by Electrophoretic Mobility Shift Analysis -- Isolation of Nuclei from Skeletal Muscle Satellite Cells and Myofibers for Use in Chromatin Immunoprecipitation Assays -- An Improved Restriction Enzyme Accessibility Assay (REAA) for Analyzing Changes in Chromatin Structure in Samples of Limited Cell Number -- ChIP Enriched in Silico Targets (ChEST), a ChIP on Chip Approach Applied to Analyzing Skeletal Muscle Genes.
520			_aOur understanding of the molecular and cellular mechanisms that control skeletal muscle development, regeneration, and adaptive responses to activity has increased dramatically in recent years, fostered by innovative techniques and approaches that are either specifically designed or adapted for research in skeletal muscle biology.  Myogenesis: Methods and Protocols presents detailed, step-by-step methods in the study of the molecular and cellular biology of skeletal muscle cells.  Protocols from different model systems including mammalian, avian, zebrafish, and invertebrate skeletal muscle are included in this volume.  Highlighted topics cover a wide range of interests and expertise including myogenic and stem cell isolation, investigation of models of exercise and disuse, viral vector delivery systems, calcium imaging, cell profiling, as well as protein-DNA and protein-protein interactions. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Comprehensive and authoritative, Myogenesis: Methods and Protocols serves as an invaluable, state-of-the-art resource for experienced and emerging scientists in basic research as well as clinical and regenerative medicine.
988			_aSpringer_Protocols_2012
650		7	_2embne _9139477 _aCitología
776	0	8	_iPrinted edition: _z9781617793424
776	0	8	_iPrinted edition: _z9781617793448
776	0	8	_iPrinted edition: _z9781493958900
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-343-1 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _ean _zSI