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008			170328s2017 xxu| fo |||| 0|eng d
020			_a9781493969210
024	7		_a10.1007/978-1-4939-6921-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH588 .S83 _b2017 EB
245	0	0	_aStem Cell Banking _bConcepts and Protocols _cedited by Jeremy M. Crook, Tenneille E. Ludwig
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (X, 210 páginas) _b18 ilustraciones, 10 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1590
505	0		_aStem Cell Banking: A Global View -- Quality Assurance in Stem Cell Banking: Emphasis on Embryonic and Induced Pluripotent Stem Cell Banking -- Acquisition and Reception of Primary Tissues, Cells, or Other Biological Specimens -- Information Management -- Cryopreservation: Vitrification and Control Rate Cooling -- Quality Assured Characterization of Stem Cells for Safety in Banking for Clinical Application -- Ethics and Governance of Stem Cell Banks -- Derivation of Human Embryonic Stem Cells -- Derivation of Human Induced Pluripotent Stem Cells in Chemically Defined Medium -- Culture, Adaptation, and Expansion of Pluripotent Stem Cells -- Cryobanking Pluripotent Stem Cells -- Genome Editing in Human Pluripotent Stem Cells -- Isolation, Culture, and Expansion of Mesenchymal Stem Cells -- Cryobanking Mesenchymal Stem Cells -- Culturing and Cryobanking Human Neural Stem Cells.
520			_aThis volume covers protocols related to both pluripotent and somatic stem cells, including the ethical procurement of tissues and cells for the provision of "seed stock," standardized methods for deriving hESCs and iPSCs, isolating mesenchymal stem cells, cell culture and cryopreservation, in addition to quality assurance and information management. Stem Cell Banking: Concepts and Protocols aims to contribute to the development of this field by providing information that is essential to establishing a bona fide stem cell bank. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Stem Cell Banking: Concepts and Protocols is a valuable resource for stem cell scientists and novices to the field, and will help strengthen and maximize their use of existing and future stem cell resources. .
988			_aSpringer_Protocols_2017
650		7	_2embne _9672226 _aBancos de tejidos
776	0	8	_iPrinted edition: _z9781493969197
776	0	8	_iPrinted edition: _z9781493969203
776	0	8	_iPrinted edition: _z9781493983391
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6921-0 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _ean _zSI