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008			140711s2014 xxu| fo |||| 0|eng d
020			_a9781493912247
024	7		_a10.1007/978-1-4939-1224-7 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP88.5 _b2014 EB
245	0	0	_aEpidermal Cells _bMethods and Protocols _cedited by Kursad Turksen
250			_a3rd edition 2014
264		1	_aNew York, NY _bSpringer International Publishing : _bImprint: Springer, _c2014
300			_a1 recurso en línea (XI, 296 páginas) _b150 ilustraciones, 34 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1195
505	0		_aDifferentiation of Human Induced Pluripotent Stem Cells into a Keratinocyte Lineage -- Differentiation of Epidermal Keratinocytes from Human Embryonic Stem Cells -- Protocol for Serial Cultivation of Epithelial Cells Without Enzymes or Chemical Compounds -- Growth and Differentiation of HaCaT Keratinocytes -- Transgene Delivery to Cultured Keratinocytes Via Replication-Deficient Adenovirus Vectors -- Analyzing the Global Chromatin Structure of Keratinocytes by MNase-Seq -- Analysis and Meta-Analysis of Transcriptional Profiling in Human Epidermis -- Compound Screening and Transcriptional Profiling in Human Primary Keratinocytes: A Brief Guideline -- Preparation of Primary Cultures of Mouse Epidermal Keratinocytes and the Measurement of Phospholipase D Activity -- Lipid Rafts and Detergent-Resistant Membranes in Epithelial Keratinocytes -- MMP-2, 9 and TIMP-1, 2 Assays in Keratinocyte Cultures -- Reactive Oxygen Species (ROS) Protection Via Cysteine Oxidation in the Epidermal Cornified Cell Envelope -- Modified Methods for Growing 3-D Skin Equivalents: An Update -- A Novel Three-Dimensional Cell Culture Method to Analyze Epidermal Cell Differentiation In Vitro -- Reconstruction of Normal and Pathological Human Epidermis on Polycarbonate Filter -- Methods for the Preparation of an Autologous Serum-Free Cultured Epidermis and for Autografting Applications -- Human Keratinocyte Cultures in the Investigation of Early Steps of Human Papillomavirus Infection -- Preparation and Delivery of 4-Hydroxy-Tamoxifen for Clonal and Polyclonal Labeling of Cells of the Surface Ectoderm, Skin, and Hair Follicle -- Microdissection and Visualization of Individual Hair Follicles for Lineage Tracing Studies -- Isolation and Characterization of a Stem Cell Side-Population from Mouse Hair Follicles -- Multi-Scale Mathematical Modeling and Simulation of Cellular Dynamical Process.
520			_aReflecting over three decades of advances, Epidermal Cells: Methods and Protocols, Third Edition underscores these advances in our understanding of epidermal biology with updated and entirely new protocols that compliment and extend the earlier edition. The inclusion of protocols useful for both in vitro and in vivo studies reflects many useful developments in the field. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Dependable and easy to follow, Epidermal Cells: Methods and Protocols, Third Edition serves researchers working to accelerate the work in this vital field of study.
988			_aSpringer_Protocols_2014
650		7	_2embne _9139477 _aCitología
776	0	8	_iPrinted edition: _z9781493912254
776	0	8	_iPrinted edition: _z9781493912230
776	0	8	_iPrinted edition: _z9781493955787
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-1224-7 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _ean _zSI