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008			100301s2009 xxu| fo |||| 0|eng d
020			_a9781597455664
024	7		_a10.1007/978-1-59745-566-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP552 .U24 _b2009 EB
245	0	0	_aSUMO Protocols _cedited by Helle Ulrich
250			_a1st edition 2009
264		1	_aTotowa, NJ _bHumana Press _c2009
300			_a1 recurso en línea (X, 324 páginas) _b70 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v497
505	0		_aThe SUMO System: An Overview -- Identification of Sumo Targets -- Identification of SUMO Target Proteins by Quantitative Proteomics -- Identification of SUMO-Conjugated Proteins and their SUMO Attachment Sites Using Proteomic Mass Spectrometry -- Identification of SUMO Targets Through In Vitro Expression Cloning -- Enhanced Detection of In Vivo SUMO Conjugation by Ubc9 Fusion-Dependent Sumoylation (UFDS) -- In Vivo Detection and Characterization of Sumoylation Targets in Saccharomyces cerevisiae -- Identification Of Sumo Interactors -- Identification of SUMO-Interacting Proteins by Yeast Two-Hybrid Analysis -- Identification of SUMO-Binding Motifs by NMR -- Functional Analysis Of Sumoylation In Vivo -- Regulation of Transcription Factor Activity by SUMO Modification -- Characterization of the Effects and Functions of Sumoylation Through Rapamycin-Mediated Heterodimerization -- Biochemical Studies Of Sumo Conjugation -- Purification of SUMO Conjugating Enzymes and Kinetic Analysis of Substrate Conjugation -- Performing In Vitro Sumoylation Reactions Using Recombinant Enzymes -- Preparation of Sumoylated Substrates for Biochemical Analysis -- Strategies for the Expression of SUMO-Modified Target Proteins in Escherichia coli -- Biochemical Studies of Sumo Deconjugation -- Preparation of SUMO Proteases and Kinetic Analysis Using Endogenous Substrates -- An In Vitro FRET-Based Assay for the Analysis of SUMO Conjugation and Isopeptidase Cleavage -- FRET-Based In Vitro Assays for the Analysis of SUMO Protease Activities -- Detection and Characterization of SUMO Protease Activity Using a Sensitive Enzyme-Based Reporter Assay -- Manipulation And Alternative Uses Of The Sumo System -- Inhibition of the SUMO Pathway by Gam1 -- SUMO Fusion Technology for Enhanced Protein Production in Prokaryotic and Eukaryotic Expression Systems.
520			_aWhile the small ubiquitin-related modifier SUMO uses a conjugation and de-conjugation system closely related to that of ubiquitin itself, its functions are highly diverse and largely independent of the ubiquitin system. In SUMO Protocols, leading experts present a collection of methods intended to highlight important features unique to the biology of SUMO in order to support future SUMO research. The first half of the volume focuses on the identification and functional characterization of SUMO targets and interactors, while the second half covers essential biochemical methods for the manipulation of the enzymatic components of the SUMO system. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include brief introductions to the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and the Notes sections, which provide tips on troubleshooting and avoiding known pitfalls. Unique and cutting-edge, SUMO Protocols provides the necessary tools for biochemists, molecular and cellular biologists as well as research-oriented clinicians not yet familiar with this vital system.
988			_aSpringer_Protocols_2009
650		7	_2embne _9139103 _aBiología molecular
776	0	8	_iPrinted edition: _z9781603279857
776	0	8	_iPrinted edition: _z9781617379451
776	0	8	_iPrinted edition: _z9781934115800
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-566-4 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _ean _zSI