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020 _a9781617792670
024 7 _a10.1007/978-1-61779-267-0
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQH588 .S83
_b2012 EB
245 0 0 _aHuman Embryonic and Induced Pluripotent Stem Cells
_bLineage-Specific Differentiation Protocols
_cedited by Kaiming Ye, Sha Jin
250 _a1st edition 2012
264 1 _aTotowa, NJ
_bHumana Press
_c2012
300 _a1 recurso en línea (XVI, 486 páginas)
_b88 ilustraciones, 48 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aSpringer Protocols Handbooks
_x1949-2456
505 0 _aFeeder-Free Growth of Undifferentiated Human Embryonic Stem Cells -- Growth of Human Embryonic Stem Cells in Long-Term Hypoxia -- Laboratory-Scale Purification of a Recombinant E-Cadherin-IgG Fc Fusion Protein that Provides a Cell Surface Matrix for Extended Culture and Efficient Subculture of Human Pluripotent Stem Cells -- Scale-Up of Single Cell-Inoculated Suspension Cultures of Human Embryonic Stem Cells -- Three-Dimensional Culture for Expansion and Differentiation of Embryonic Stem Cells -- Expansion of Pluripotent Stem Cells in Defined Xeno-Free Culture System -- Pluripotent Stem Cells In Vitro from Human Primordial Germ Cells -- Cryopreservation of Human Embryonic Stem Cells and Induced Pluripotent Stem Cells -- Induced Pluripotent Stem Cells (iPSC) from Cord Blood CD133+ Cells Using Oct4 and Sox2 -- Generation, Maintenance, and Differentiation of Human iPS Cells from Cord Blood -- Generation of iPS Cells from Human Umbilical Vein Endothelial Cells by Lentiviral Transduction and Their Differentiation to Neuronal Lineage -- Generation of Human Induced Pluripotent Stem Cells from Endoderm Origin Cells -- Derivation of Human Induced Pluripotent Stem Cells on Autologous Feeders -- Human Mesenchymal Stem Cells and iPS Cells (Preparation Methods) -- Retroviral Vector-Based Approaches for the Generation of Human Induced Pluripotent Stem Cells from Fibroblasts and Keratinocytes -- Generation of Nonviral Integration-Free Induced Pluripotent Stem Cells from Plucked Human Hair Follicles -- Generation of iPS Cells from Human Skin Biopsy -- Generation of Induced Pluripotent Stem Cells from Human Amnion Cells -- In Vitro Two-Dimensional Endothelial Differentiation of Human Embryonic Stem Cells -- Feeder-Free Culture for High Efficiency Production of Subculturable Vascular Endothelial Cells from Human Embryonic Stem Cells -- Feeder-Independent Maintenance of Human Embryonic Stem Cells and Directed Differentiation into Endothelial Cells under Hypoxic Condition -- Differentiation of Endothelial Cells from Human Embryonic Stem Cells and Induced Pluripotent Stem Cells -- Differentiation of Human Embryonic and Induced Pluripotent Stem Cells into Blood Cells in Coculture with Murine Stromal Cells -- Generation of Multipotent CD34+CD45+ Hematopoietic Progenitors from Human Induced Pluripotent Stem Cells -- Adipogenic Differentiation of Human Induced Pluripotent Stem Cells -- Chondrogenic Differentiation of hESC in Micromass Culture -- Deriving Hepatic Endoderm from Pluripotent Stem Cells -- Multistage Hepatic Differentiation from Human Induced Pluripotent Stem Cells -- Hepatic Maturation of hES Cells by Using a Murine Mesenchymal Cell Line Derived from Fetal Livers -- Generation of Lung Epithelial-Like Tissue from hESC by Air-Liquid Interface Culture -- Direct Differentiation of Human Embryonic Stem Cells into Selective Neurons on Nanoscale Ridge/Groove Pattern Arrays -- Neural Differentiation of Human ES and iPS Cells in Three-Dimensional Collagen and MatrigelTM Gels -- Single-Cell Transcript Profiling of Differentiating Embryonic Stem Cells -- Using Endogenous MicroRNA Expression Patterns to Visualize Neural Differentiation of Human Pluripotent Stem Cells.
520 _aBecause of the huge potential of human embryonic stem (hES) cells, especially the newly developed human induced pluripotent stem (hiPS) cells, in disease treatment and life quality improvement, enormous efforts have been made to develop new methodologies to translate lab discoveries in stem cell research into bed-side clinical technologies. In Human Embryonic and Induced Pluripotent Stem Cells: Lineage-Specific Differentiation Protocols, experts in the field present a comprehensive collection of protocols designed for labs around the world.  The topics covered in this detailed volume include techniques used for maintenance of hES and iPS cells in either small or large scale, techniques for directing hES and iPS cell lineage specification, techniques for enhancing the maturity of differentiated hES and iPS cells within three-dimensional scaffolds, techniques for reprogramming adult cells into iPS cells, techniques for generating patient-specific iPS cells, and techniques for translating hES and iPS cell research into new therapies.  Chapters include lab ready protocols with tips on troubleshooting and avoiding known pitfalls.   Wide-ranging and authoritative, Human Embryonic and Induced Pluripotent Stem Cells: Lineage-Specific Differentiation Protocols will be a tremendous aid for researchers and students who wish to explore these areas and transform their discoveries into the next generation of regenerative medicine and tissue engineering technologies.
988 _aSpringer_Protocols_2012
650 7 _2embne
_9160528
_aCélulas madre
776 0 8 _iPrinted edition:
_z9781617792663
776 0 8 _iPrinted edition:
_z9781617792687
776 0 8 _iPrinted edition:
_z9781493958931
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-267-0
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b12/2023
_dz
_ean
_zSI