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008			100301s1998 xxu| fo |||| 0|eng d
020			_a9781592595778
024	7		_a10.1385/0896035204 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP519.9 .B55 _b1998 EB
245	0	0	_aBioluminescence Methods and Protocols _cedited by Robert A. LaRossa
250			_a1st edition 1998
264		1	_aTotowa, NJ _bHumana Press _c1998
300			_a1 recurso en línea (X, 310 páginas) _b33 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v102
505	0		_aThe Basics -- Improvements in the Application of Firefly Luciferase Assays -- Visualization of Bioluminescence -- Microscopic Imagery of Mammalian Cells Expressing an Enhanced Green Fluorescent Protein Gone -- Analytical Biochemistry -- Luminometric Measurement of Malate and Glucose-6-Phosphate in Mammalian Tissue -- Bioluminescent Assay of the Guanylates -- Bioluminescent Assay of the Adenylate Energy Charge -- Molecular Biology Tools -- Photorhabdus luminescens luxCDABE Promoter Probe Vectors -- Insertion of Promoter Region::luxCDABE Fusions into the Escherichia coil Chromosome -- Probing for Promoters with Luciferase-Transposons -- Cryopreservation and Reawakening -- Luciferase Renaturation Assays of Chaperones and Chaperone Antagonists -- Cell-Based Assays -- Genotoxic Sensors -- Stress Detection Using Bioluminescent Reporters of the Heat-Shock Response -- Real-Time Reporter of Protein Synthesis Inhibition -- Luminescence-Based Cell Viability Testing -- Phagocyte Chemiluminescence -- Detection of Oxidants Using lux Fusions to Oxidative Stress Promoters -- Environmental Applications -- Luciferase-Based Measurement of Water Contaminants -- Bioluminescence-Based Metal Detectors -- Luminescence Facilitated Detection of Bioavailable Mercury in Natural Waters -- A Panel of Stress-Responsive Luminous Bacteria for Monitoring Wastewater Toxicity -- Organic Contaminant Detection and Biodegradation Characteristics -- Detection of Firefly Luciferase-Tagged Bacteria in Environmental Samples -- Monitoring of GFP-Tagged Bacterial Cells.
520			_aComprehensive and state-of-the-art, Bioluminescence Methods and Protocols brings together a collection of innovative methods for the use of luciferase and green fluorescent protein in analytical biochemistry, molecular toxicology, environmental monitoring, and cell-based assays. The contributors-all hands-on researchers who have perfected their protcols-illustrate these powerful methods with a wide variety of applications ranging from the analytical determination (titer) of specific molecules to the cellular detection of a wide range of pollutants, to microbiological detection and analysis, and the molecular biological manipulation of lux, luc, and gfp structural genes. Step-by-step methods for the construction of other recombinant organisms are also included. Bioluminescence Methods and Protocols represents the state-of-the-art in luminescent assays. Its cutting-edge techniques, diversely illustrated applications, and detailed instructions will make it the standard reference for today's investigators who want to adapt established techniques to their purposes or apply them to a wide range of clinical, microbiological, environmental, toxicological, and analytical problems.
988			_aSpringer_Protocols_1998
650		7	_2embne _9671791 _aLuminiscencia
776	0	8	_iPrinted edition: _z9781489942654
776	0	8	_iPrinted edition: _z9780896035201
776	0	8	_iPrinted edition: _z9781489942647
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896035204 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _ean _zSI