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008			100301s2000 xxu| fo |||| 0|eng d
020			_a9781603272612
024	7		_a10.1007/978-1-60327-261-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP519.7 _b2000 EB
245	0	0	_aAffinity Chromatography : _bMethods and Protocols _cedited by Pascal Bailon
250			_a1st edition 2000
264		1	_aTotowa, NJ _bHumana Press _c2000
300			_a1 recurso en línea (X, 230 páginas) _b39 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v147
505	0		_aAn Overview of Affinity Chromatography -- Weak Affinity Chromatography -- Fluidized-Bed Receptor-Affinity Chromatography -- Site-Specific Immobilization of Antibodies to Protein G-Derivatized Solid Supports -- Affinity Purification of Monoclonal Antibodies -- Protein A Mimetic (PAM) Affinity Chromatography -- Periodate Oxidation of Antibodies for Site-Selective Immobilization in Immunoaffinity Chromatography -- Mini-Antibody Affinity Chromatography of Lysozyme -- Nitrocellulose-Based Immunoaffinity Chromatography -- Immunoaffinity Chromatography -- Affinity Partitioning of Proteins -- Boronate Affinity Chromatography -- Dye-Ligand Affinity Chromatography for Protein Separation and Purification -- DNA Affinity Chromatography -- Affinity Chromatography of Pyrogens -- Western Cross Blot -- Affinity Perfusion Chromatography -- Phage Display Technology -- Phage Display Technology.
520			_aAffinity chromatography, with its exquisite specificity, is based upon molecular recognition. It is a powerful tool for the purification of biomolecules. In recent years, numerous new applications and modified techniques have been derived from gro- specific interactions and biological recognition principles. An up-to-date review of the past, current, and future applications of affinity chromatography has been presented in the introductory chapter by Meir Wilchek and Irwin Chaiken. Though many of these new applications and techniques are well documented in the literature, it is often difficult to find methods that are written with the intent of helping new practitioners of affinity chromatography. This volume on Affinity Chromatography: Methods and Protocols is intended for the novice, as well as for - perts in the field. The protocols are written by experts who have developed and/or successfully employed these methods in their laboratories. Each chapter describes a specific technique, and since the book is intended to help the beginner, each technique is described simply and clearly, making sure that all relevant steps are included, assuming no previous knowledge. Each chapter contains an introduction describing the principles involved, followed by a Materials and Methods section, which lays the groundwork for the reader to conduct experiments step-by-step, in an orderly fashion. The following Notes section, which describes many of the problems that can occur, makes suggestions for overcoming them, and provides alternate procedures. These are precisely the sort of important, practical details that never seem to appear in the published literature.
988			_aSpringer_Protocols_2000
650		7	_2embne _9149316 _aBioquímica clínica
650		7	_2embne _9683072 _aCromatografía
776	0	8	_iPrinted edition: _z9781489941947
776	0	8	_iPrinted edition: _z9781489941930
776	0	8	_iPrinted edition: _z9780896036949
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60327-261-2 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _ean _zSI