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020 _a9781493907588
024 7 _a10.1007/978-1-4939-0758-8
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aAnimal Influenza Virus
_cedited by Erica Spackman.
250 _a2nd edition 2014
264 1 _aNew York, NY
_bSpringer International Publishing
_c2014
300 _a1 recurso en línea (XII, 425 páginas)
_b58 ilustraciones, 48 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1161
505 0 _aHemagglutination Assay for Influenza Virus -- Hemagglutination-Inhibition Assay for Influenza Virus Subtype Identification and the Detection and Quantitation of Serum Antibodies to Influenza Virus -- Neuraminidase-Inhibition Assay for the Identification of Influenza A Virus Neuraminidase Virus Subtype or Neuraminidase Antibody Specificity -- Reverse Genetics of  Influenza Virus -- Immunohistochemical Staining of Influenza Virus in Tissues -- A Brief Introduction to Avian Influenza Virus -- Wild Bird Surveillance for Avian Influenza Virus -- Avian Influenza Virus Sample Types, Collection and Handling -- Avian Influenza Virus RNA Extraction -- Avian Influenza Virus Detection and Quantitation by Real-Time RT-PCR -- Influenza Subtype Identification with Molecular Methods -- Avian Influenza Virus Isolation, Propagation and Titration in Embryonated Chicken Eggs -- Agar Gel Immunodiffusion Assay to Detect Antibodies to Type A Influenza Virus -- Detection of Influenza A Antibodies in Avian Serum Samples by ELISA -- Conducting Influenza Virus Pathogenesis Studies in Avian Species -- Laboratory Methods for Assessing and Licensing Influenza Vaccines for Poultry -- Detection of Cell Mediated Immune Response to Avian Influenza Viruses -- Characterization of Cytokine Expression Induced by Avian Influenza Virus Infection with Real-Time RT-PCR -- Newcastle Disease Virus Detection and Differentiation from Avian Influenza -- A Brief Introduction to Influenza A Virus in Swine -- Sample Types, Collection, and Transport for Influenza A Viruses of Swine -- Isolation of Swine Influenza Virus in Cell Cultures and Embryonated Chicken Eggs -- RNA Extraction from Swine Samples and Detection of Influenza A Virus in Swine by Real-Time RT-PCR -- Hemagglutinin Inhibition Assay with Swine Sera -- Enzyme-Linked Immunosorbent Assay for Detection of Serum or Mucosal Isotype Specific IgG and IgA Whole Virus Antibody to Influenza A Virus in Swine -- Serum Virus Neutralization Assay for Detection and Quantitation of Serum Neutralizing Antibodies to Influenza A Virus in Swine -- Microneutralization Assay for Swine Influenza Virus in Swine Serum -- Analyzing Swine Sera for Functional Antibody Titers Against Influenza A Neuraminidase Proteins Using an Enzyme-Linked Lectin Assay (ELLA) -- Antibody Secreting Cell Assay for Influenza A Virus in Swine -- In vivo Models for Pathotyping and Vaccine Efficacy for Swine Influenza -- A Brief Introduction to Equine Influenza and Equine Influenza Viruses -- Equine Influenza Diagnosis: Sample Collection and Transport -- RNA Extraction from Equine Samples for Equine Influenza Virus -- Type A Influenza Virus Detection from Horses by Real-Time RT-PCR and Insulated Isothermal RT-PCR -- Equine Influenza Culture Methods -- Equine Influenza Serological Methods.
520 _aAvian Influenza Virus, Second Edition aims to provide the essential methods used in working with animal influenza viruses, and to compile more advanced information that will guide the user in designing influenza studies. Influenza A viruses are among the most important pathogens for humans, food animals and companion animals. Research and diagnostics with animal influenza viruses are critical to animal health and it should be recognized that the needs and goals of animal agriculture and veterinary medicine are not always the same as those of public health.  This volume sorts out the differences in the structure of the poultry, swine and equine industries, the biological differences of influenza virus from each animal group, and provides host, strain and lineage specific guidance and procedures. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and accessible, Avian Influenza Virus, Second Edition seeks to serve both professionals and novices with its well-honed methodologies in an effort to further our knowledge of these important pathogens.
700 1 _aSpackman, Erica
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781493907571
776 0 8 _iPrinted edition:
_z9781493907595
776 0 8 _iPrinted edition:
_z9781493947560
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-0758-8
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2014
999 _c392950
_d392950