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020 _a9781597455718
024 7 _a10.1007/978-1-59745-571-8
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aVascular Biology Protocols
_cedited by Nair Sreejayan, Jun Ren.
250 _a1st edition 2007
264 1 _aTotowa, NJ
_bHumana Press
_c2007
300 _a1 recurso en línea (XII, 372 páginas)
_b
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Medicine
_x1940-6037
_v139
505 0 _aRat Carotid Artery Balloon Injury Model -- Histological and Morphometric Analyses for Rat Carotid Balloon Injury Model -- Plaque Rupture Model in Mice -- Immunostaining of Mouse Atherosclerotic Lesions -- Surgical Animal Model of Ventricular Hypertrophy -- Animal Models of Hypertension -- Rat Models of Cardiac Insulin Resistance -- The Isolated, Perfused Pseudo-Working Heart Model -- Altering and Analyzing Glucose Metabolism in Perfused Hearts of Transgenic Mice -- Methods in the Evaluation of Cardiovascular Renin Angiotensin Aldosterone Activation and Oxidative Stress -- Detection and Quantification of Apoptosis in the Vasculature -- Endothelial Progenitor Cell and Mesenchymal Stem Cell Isolation, Characterization, Viral Transduction -- Assessment of Endothelial Damage/Dysfunction: A Focus on Circulating Endothelial Cells -- Evaluation of Endothelial Function by In Vivo Microscopy -- Haptotaxis of Endothelial Cell Migration Under Flow -- Isolation, Culture, and Functional Analysis of Adult Mouse Cardiomyocytes -- Mechanical Measurement of Contractile Function of Isolated Ventricular Myocytes -- Studying Ischemia and Reperfusion in Isolated Neonatal Rat Ventricular Myocytes Using Coverslip Hypoxia -- Isolation and Functional Studies of Rat Aortic Smooth Muscle Cells -- Detection of Reactive Oxygen Species and Nitric Oxide in Vascular Cells and Tissues -- Assessment of Protein Glycoxidation in Ventricular Tissues -- Assessment of PI-3 Kinase and Akt in Ischemic Heart Diseases in Diabetes -- Two-Dimensional Gel Electrophoresis in Platelet Proteomics Research -- Stem Cell Therapy in the Heart and Vasculature.
520 _aOver the past decades, the pathogenesis, diagnosis, treatment and prevention of cardiovascular diseases have been benefited significantly from intensive research activities. In order to provide a comprehensive "manual" in a field that has become as broad and deep as cardiovascular medicine, Vascular Biology Protocols covers a wide spectrum of in vivo and in vitro techniques encompassing biochemical, pharmacological and molecular biology disciplines which are currently used to assess vascular disease progression. Each chapter included in this volume focuses on a specific vascular biology technique and describes various applications as well as caveats of these techniques. The protocols included here are described in detail, allowing beginners with little experience in the field of vascular biology to embark on new research projects. Comprehensive and easy-to-use, Vascular Biology Protocols will prove valuable for all those who wish to broaden their knowledge of cardiovascular research tools.
700 1 _aSreejayan, Nair
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aRen, Jun
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781617376504
776 0 8 _iPrinted edition:
_z9781603278058
776 0 8 _iPrinted edition:
_z9781588295743
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-571-8
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2007
999 _c392944
_d392944