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008			110414s2009 xxu| s |||| 0|eng d
020			_a9781597455381
024	7		_a10.1007/978-1-59745-538-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aDNA Microarrays for Biomedical Research _bMethods and Protocols _cedited by Martin Dufva.
250			_a1st edition 2009
264		1	_aTotowa, NJ _bHumana Press _c2009
300			_a1 recurso en línea (XII, 252 páginas) _b54 ilustraciones, 1 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v529
505	0		_ato Microarray Technology -- Probe Design for Expression Arrays Using OligoWiz -- Comparative Genomic Hybridization: Microarray Design and Data Interpretation -- Design of Tag SNP Whole Genome Genotyping Arrays -- Fabrication of DNA Microarray -- Immobilization Chemistries -- Fabrication Using Contact Spotter -- RNA Preparation and Characterization for Gene Expression Studies -- Gene Expression Analysis Using Agilent DNA Microarrays -- Target Preparation for Genotyping Specific Genes or Gene Segments -- Genotyping of Mutations in the Beta-Globin Gene Using Allele Specific Hybridization -- Microarray Temperature Optimization Using Hybridization Kinetics -- Whole-Genome Genotyping on Bead Arrays -- Genotyping Single Nucleotide Polymorphisms by Multiplex Minisequencing Using Tag-Arrays -- Resequencing Arrays for Diagnostics of Respiratory Pathogens -- Comparative Genomic Hybridization: DNA Preparation for Microarray Fabrication -- Comparative Genomic Hybridization: DNA Labeling, Hybridization and Detection -- Chromatin Immunoprecipitation Using Microarrays.
520			_aDNA microarray technology has revolutionized research in the past decade. Initially an application for mRNA expression studies, the technology now has spread to other applications such as comparative genomic hybridization, SNP and mutation analysis. In DNA Microarrays for Biomedical Research: Methods and Protocols, experts explore these now commonly used applications, addressing probe design strategies, fabrication issues and providing practical examples of detailed methods for generation of high quality DNA microarray data. Chapters incorporate information on some of the largest providers of microarray, including Affymetrix, Ilumina and Agilent, and their use on a variety of applications. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Authoritative and highly practical, DNA Microarrays for Biomedical Research: Methods and Protocols presents a variety of protocols which can be easily reproduced, allowing researchers to gain surprising insight into the complex world of DNA microarray technology.
700	1		_aDufva, Martin _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781607612247
776	0	8	_iPrinted edition: _z9781617379413
776	0	8	_iPrinted edition: _z9781934115695
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-538-1 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
988			_aSpringer_Protocols_2009
999			_c392931 _d392931