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008			170227s2017 xxu| s |||| 0|eng d
020			_a9781493968206
024	7		_a10.1007/978-1-4939-6820-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aThermogenic Fat _bMethods and Protocols _cedited by Jun Wu.
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XI, 214 páginas) _b42 ilustraciones, 30 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1566
505	0		_aIsolation, Primary Culture, and Differentiation of Preadipocytes from Mouse Brown Adipose Tissue -- Isolation of Mouse Stromal Vascular Cells for Monolayer Culture -- Flow Cytometry Assisted Isolation of Adipose Tissue Derived Stem Cells -- Flow Cytometric Isolation and Differentiation of Adipogenic Progenitor Cells into Brown and Brite/Beige Adipocytes -- Immuno-Magnetic Isolation and Thermogenic Differentiation of White Adipose Tissue Progenitor Cells -- Isolation of Immune Cells from Adipose Tissue -- Differentiation and Metabolic Interrogation of Human Adipocytes -- Protocols for Generation of Immortalized Human Brown and White Preadipocyte Cell Lines -- Gene Expression and Histological Analysis of Activated Brown Adipocytes in Adipose Tissue -- Genetic Mouse Models: The Powerful Tools to Study Fat Tissues -- Genetic Manipulation with Viral Vectors to Assess Metabolism and Adipose Tissue Function -- Bioenergetic Analyses in Adipose Tissue -- Oxygen Consumption Rate and Energy Expenditure in Mice: Indirect Calorimetry -- Isolation and Patch-Clamp of Primary Adipocytes -- In Vitro Approaches to Model and Study Communications Between Adipose Tissue and the Liver -- Identification and Quantification of Human Brown Adipose Tissue -- Designing 3D Adipospheres for Quantitative Metabolic Study -- Culture and Sampling of Primary Adipose Tissue in Practical Microfluidic Systems -- Using Thermogenic Beige Cells to Identify Biologically Active Small Molecules and Peptides.
520			_aThis volume presents a collection of protocols that describe methodologies to study thermogenic fat biology from various angles. This book is divided into 2 parts. Part 1 focuses on establishing in vitro culture systems. The chapters in this section introduce techniques on how to isolate, culture, and differentiate primary fat cells from both laboratory mice and humans. This part also presents flow cytometry methods to isolate various subpopulations of precursors within the stromal vascular fraction of the adipose tissue, which contains both preadipocytes and immune cells. Part 2 introduces multiple means to genetically manipulate and evaluate brown and beige fat in vivo. The chapters in this section explore methods on bioenergetics analyses both in vitro and in vivo. They also cover how to evaluate thermogenic fat contents and activity in humans, how to culture these cells though interdisciplinary approaches, and how to use thermogenic fat cell lines to carry out drug screens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Thermogenic Fat: Methods and Protocols is a valuable resource for both experts and novices who are interested in learning assays and investigating brown and beige fat functions.
700	1		_aWu, Jun _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493968190
776	0	8	_iPrinted edition: _z9781493968213
776	0	8	_iPrinted edition: _z9781493983087
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6820-6 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c392911 _d392911