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020			_a9781592592456
024	7		_a10.1385/1592592457 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aAntiviral Methods and Protocols _cedited by Derek Kinchington, Raymond F. Schinazi.
250			_a1st edition 2000
264		1	_aTotowa, NJ _bHumana Press _c2000
300			_a1 recurso en línea (XIV, 404 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Medicine _x1940-6037 _v24
505	0		_aChanging Methods for Discovering Antiviral Drugs -- Changing Methods for Discovering Antiviral Drugs -- Laboratory Safety Considerations -- Laboratory Safety Considerations -- Routine Quality Control Testing of Cell Cultures -- Routine Quality Control Testing of Cell Cultures -- Hepatitis Virus -- Hep AD38 Assay -- Hepatitis B Virus Cell Culture Assays for Antiviral Activity -- A Rapid Microtiter Assay for Duck Hepatitis Virus Reverse Transcriptase -- Assays for Measuring Hepadnaviral Supercoiled DNA -- Assays for Hepatitis B Virus DNA-and RNA-Dependent DNA Polymerase Activities -- Development of a Hepatitis C Virus RNA Helicase High Throughput Assay -- Herpesvirus -- Determination of Viral Infectivity -- Antiviral Screening Assays for Human Herpesviruses 6 and 7 -- Strategies for Evaluation of Antiviral Agents Against Epstein-Barr Virus in Culture -- Monitoring Drug Resistance for Herpesviruses -- Herpesvirus Protease Assays -- Human Immunodeficiency Virus -- Testing Compounds for Antiviral Activity in Cell Cultures Infected with HIV -- Evaluation of Compounds That Prevent Reactivation of HIV-1 in OM-10.1 Cells -- Assessment of Activity of Topical Virucidal Agents -- Assays That Measure Selective Killing of Virus-Infected Cells -- Evaluating Clinical Isolates for Their Phenotypic and Genotypic Resistance Against Anti-HIV Drugs -- Line Probe Assay for Detecting Mutations in HIV-1 Reverse Transcriptase -- RT-PCR for Amplification of Specific Fragments of HIV-1 Genome -- Evaluation of Compounds Against Recombinant HIV Reverse Transcriptase -- Ultrasensitive Detection of Reverse Transcriptase Activity by the Amp-RT Assay -- Reverse Transcriptase Assay Based on Product Enhancement for Assessing the Drug Susceptibility of Retroviruses -- Identifying and Characterizing HIV Protease Inhibitors -- Retroviral Integrase -- Human Papilloma Virus -- Papillomavirus DNA Replication -- Quantitative Immunodetection of Viral Oncoprotein Levels in Human Papillomavirus-Transformed Human Cells -- Influenza Virus -- Biochemical Methods for the Characterization of Influenza Viruses with Reduced Sensitivity to 4-Guanidino-Neu5Ac2en -- Virological Methods for the Generation and Characterization of Influenza Viruses with Reduced Sensitivity to 4-Guanidino-Neu5Ac2en.
520			_aIn Antiviral Methods and Protocols, experienced scientists describe-in an easily-followed format-their state-of-the-art techniques for evaluating antiviral compounds. The assays described include systems for investigating drugs used against herpesviruses, hepatitis viruses, human immunodeficiency viruses, human papillomaviruses, and influenza viruses. These well-tested methods range from cellular assays to some of the most up-to-date molecular approaches for identifying compounds that are active against viral enzymes and the development of viral resistance against drugs currently in use. The detailed protocols also discuss possible pitfalls and ways to overcome them. Timely and comprehensive, Antiviral Methods and Protocols offers today's researchers in academia, clinical departments, and the pharmaceutical industry the powerful, reproducible, and novel methods needed to evaluate compounds effective against both acute and chronic infections.
700	1		_aKinchington, Derek _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aSchinazi, Raymond F _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617370731
776	0	8	_iPrinted edition: _z9781489941657
776	0	8	_iPrinted edition: _z9780896035614
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592592457 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
988			_aSpringer_Protocols_2000
999			_c392910 _d392910