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020 _a9781607617112
024 7 _a10.1007/978-1-60761-711-2
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 4 _aThe Urinary Proteome
_bMethods and Protocols
_cedited by Alex J. Rai.
250 _a1st edition 2010
264 1 _aTotowa, NJ
_bHumana Press
_c2010
300 _a1 recurso en línea (VI, 358 páginas)
_b82 ilustraciones, 4 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v641
505 0 _ato Urinalysis: Historical Perspectives and Clinical Application -- A Primer on Clinical Applications and Assays Using Urine: Focus on Analysis of Plasma Cell Dyscrasias Using Automated Electrophoresis and Immunofixation -- Application of Free Flow Electrophoresis to the Analysis of the Urine Proteome -- Standardized Preprocessing of Urine for Proteome Analysis -- Different Sample Preparation and Detection Methods for Normal and Lung Cancer Urinary Proteome Analysis -- Isolation and Purification of Exosomes in Urine -- Bioinformatics of the Urinary Proteome -- PROTIS: Use of Combined Biomarkers for Providing Diagnostic Information on Disease States -- Statistical Contributions to Proteomic Research -- A Review of Experimental Design Best Practices for Proteomics Based Biomarker Discovery: Focus on SELDI-TOF -- Urine Proteomic Profiling for Biomarkers of Acute Renal Transplant Rejection -- Surface Plasmon Resonance Biosensorics in Urine Proteomics -- Urinary Proteins for the Diagnosis of Obstructive Sleep Apnea Syndrome -- Immune Response Biomarker Profiling Application on ProtoArray® Protein Microarrays -- Design and Validation of an Immunoaffinity LC-MS/MS Assay for the Quantification of a Collagen Type II Neoepitope Peptide in Human Urine: Application as a Biomarker of Osteoarthritis -- Cell-Specific Biomarkers in Renal Medicine and Research -- Proteomic Assays for the Detection of Urothelial Cancer -- Urine Proteomic Analysis: Use of Two-Dimensional Gel Electrophoresis, Isotope Coded Affinity Tags, and Capillary Electrophoresis -- Proteomic Analysis of Pancreatic Secretory Trypsin Inhibitor/Tumor-Associated Trypsin Inhibitor from Urine of Patients with Pancreatitis or Prostate Cancer.
520 _aUrine is one of the most easily accessible biological samples, and it provides a treasure trove of molecules essential for clinical diagnostics. In The Urinary Proteome: Methods and Protocols, expert researchers review briefly the classical urine tests that are performed in the clinical laboratory and then delve into the state of the art methods for proteomic analysis using urine specimens. Featuring the most recent advances in sample preparation, data analysis, and methods and applications, the book contains a multitude of detailed methods, including procedural details for the identification and characterization of urine biomarkers, which hold great potential for the diagnosis and treatment of many different disease conditions. Written in the highly successful Methods in Molecular Biology™ series format, chapters present brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls. Authoritative and systematic, The Urinary Proteome: Methods and Protocols is an ideal guide for scientific researchers, clinical laboratorians, clinical and translational scientists, and others interested in the vital study of proteomics and biomarker discovery.
700 1 _aRai, Alex J
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781607617174
776 0 8 _iPrinted edition:
_z9781607617105
776 0 8 _iPrinted edition:
_z9781493957385
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60761-711-2
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2010
999 _c392909
_d392909