000			03811nam a22003735i 4500
001			392885
003			ES-MaUEC
005			20230102122852.0
007			cr nn 008mamaa
008			100301s2006 xxu| s |||| 0|eng d
020			_a9781592599868
024	7		_a10.1385/1592599869 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aHormone Assays in Biological Fluids _cedited by Michael J. Wheeler, William D. Fraser, J. S. Morley Hutchinson.
250			_a1st edition 2006
264		1	_aTotowa, NJ _bHumana Press _c2006
300			_a1 recurso en línea (IX, 258 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v324
505	0		_aImmunoassay Techniques -- High-Performance Liquid Chromatography for Hormone Assay -- Gas Chromatography-Mass Spectrometry -- An Introduction to PCR Techniques -- Methods for the Investigation of Thyroid Function -- Assays for LH, FSH, and Prolactin -- Adrenocorticotrophic Hormone and Related Peptides -- Measurement of Glucocorticoids in Biological Fluids -- Urinary Steroid Profiling -- Measurement of Aldosterone in Blood -- Measurement of Plasma Renin Activity -- Measurement of Androgens -- Measurement of Gut Hormones in Plasma -- Measurement of Melatonin and 6-Sulphatoxymelatonin.
520			_aAlthough automated systems can measure all common hormones in human blood, they are expensive and often incapable of measuring hormones in urine, saliva, and animals. In Hormone Assays in Biological Fluids, expert researchers who have developed and applied significant new assays describe in step-by-step detail readily reproducible methods for measuring a broad variety of hormones, related peptides, and synthetic steroids in various biological fluids. The hormones measured range from glucocorticoids in biological fluids, urinary steroids, aldosterone in blood, and plasma renin activity, to gut hormones in plasma, melatonin, prolactin, 6-sulfatoxymelatonin, and androgens in blood, saliva, and hair. The emphasis is on noncommercial assays so that investigators can develop novel methods suited to their special needs. Commercial assays are also described for comparative purposes. Tutorials on radioimmunoassay, gas chromatography-mass spectrometry, high-performance liquid chromatography, and polymerase chain reaction techniques help readers choose the best methods for their purpose. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Wide-ranging and highly practical, Hormone Assays in Biological Fluids illuminates the many different methods available for a broad range of hormone assays, enabling researchers to choose the optimal existing methods or develop novel assays for their specialized purposes.
700	1		_aWheeler, Michael J _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aFraser, William D _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aHutchinson, J. S. Morley _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617372995
776	0	8	_iPrinted edition: _z9781588290052
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592599869 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
988			_aSpringer_Protocols_2006
999			_c392885 _d392885