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008			100301s2002 xxu| fo |||| 0|eng d
020			_a9781592591893
024	7		_a10.1385/1592591892 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP606 .T44 _b2002 EB
245	0	0	_aTelomeres and Telomerase _bMethods and Protocols _cedited by John A. Double, Michael J. Thompson
250			_a1st edition 2002
264		1	_aTotowa, NJ _bHumana Press _c2002
300			_a1 recurso en línea (XII, 224 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v191
505	0		_ato Telomeres and Telomerase -- Detection of Chromosome Ends by Telomere FISH -- Telomere Length Distribution -- Analysis of Telomerase RNA Gene Expression by In Situ Hybridization -- Relative Gene Expression in Normal and Tumor Tissue by Quantitative RT-PCR -- Quantitative Detection of Telomerase Components by Real-Time, Online RT-PCR Analysis with the LightCycler -- Standard TRAP Assay -- Stretch PCR Assay -- Fluorescent Detection of Telomerase Activity -- Nonradioactive Detection of Telomerase Activity Using a PCR-ELISA-Based Telomeric Repeat Amplification Protocol -- In Situ TRAP Assay Detection of Telomerase Activity in Cytological Preparations -- Biotinylated Primer for Detecting Telomerase Activity Without Amplification -- Whole-Cell and Microcell Fusion for the Identification of Natural Regulators of Telomerase -- Screening with COMPARE Analysis forTelomerase Inhibitors -- Telomerase as a Therapeutic Target.
520			_aThe discovery of telomerase expression in a majority of tumors and the recognition of its potential role in the aging process has dramatically raised its profile as a target for therapeutic intervention. In Telomeres and Telomerase: Methods and Protocols, John A. Double and Michael J. Thompson have collected a critically important series of novel and essential techniques for studying telomeres and telomerase. These readily reproducible protocols provide cutting-edge tools to identify, measure, and analyze telomeres, to determine telomerase expression at the RNA level, to determine telomerase activity, and to detect potential modifiers of this activity. The techniques for assaying telomerase activity range from standard radiological TRAP assays to nonradioactive methods, from non-PCR-based methods to techniques using real-time PCR. Written by investigators who have used the methods extensively, each protocol includes step-by-step instructions, tips on avoiding pitfalls, and invaluable notes that make all the difference to successful experimental outcomes. Broad-ranging and highly practical, Telomeres and Telomerase: Methods and Protocols provides molecular and cell biologists, cancer researchers, and cellular pathologists with the core array of productive techniques needed today to develop telomerase inhibitors and clinically valuable diagnostic/prognostic telomerase markers.
988			_aSpringer_Protocols_2002
650		7	_2embne _9688615 _aTelómeros
776	0	8	_iPrinted edition: _z9781617371189
776	0	8	_iPrinted edition: _z9781489938619
776	0	8	_iPrinted edition: _z9780896036574
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592591892 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b11/2023 _dz _ean _zSI