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008			100301s2000 xxu| fo |||| 0|eng d
020			_a9781592590766
024	7		_a10.1385/1592590764 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aRM282 .M65 _b2000 EB
245	0	0	_aDiagnostic and Therapeutic Antibodies _cedited by Andrew J.T. George, Catherine E. Urch
250			_a1st edition 2000
264		1	_aTotowa, NJ _bHumana Press _c2000
300			_a1 recurso en línea (XIV, 477 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Medicine _x1940-6037 _v40
505	0		_aThe Antibody Molecule -- Polyclonal and Monoclonal Antibodies -- Engineering Antibody Molecules -- Phage Display Technology -- Antibodies in Medicine -- Prospects for the Application of Antibodies in Medicine -- Antibodies for Neoplastic Disease -- The Application of Monoclonal Antibodies in the Treatment of Lymphoma -- Antibodies for Inflammatory Disease -- Antibodies for Inflammatory Disease -- Antibodies for Transplantation -- Antibody-Based Therapies in Infectious Diseases -- Antibodies in Nuclear Medicine -- Animal Models for Tumor Localization -- Antibodies for Immunoassays -- Ethics and Industry -- Intellectual Property -- From Laboratory to Clinic -- Production and Purification -- Production of Monoclonal Antibodies -- Purification of Monoclonal Antibodies Using Protein A/G -- Preparation of Monoclonal Antibodies Using Ion Exchange Chromatography -- Quality Control of Raw Materials -- Cell Banks and Stability of Antibody Production -- Measurement of Endotoxin -- Aseptic Vial Filling -- Measurement of Antibody Concentrations by Hemagglutination -- Modification of Antibodies -- Enzymatic Digestion of Monoclonal Antibodies -- How to Make Bispecific Antibodies -- Radiolabeling of Monoclonal Antibodies -- Determination of the Immunoreactivity of Radiolabeled Monoclonal Antibodies -- Use of Biosensors to Measure the Kinetics of Antibody-Antigen Interactions -- Application of Antibodies in Vitro -- How to Set Up an ELISA -- Measurement of HAMA and Anti-Idiotypic Antibodies -- SDS-PAGE and Western Blotting -- Flow Cytometric Analysis -- Immunocytochemistry -- Immunolabeling for Electron Microscopy -- Antibody Engineering -- PCR of the V Region -- Phage Display Technology.
520			_aThe use of monoclonal antibodies in treating disease has long held tantalizing promise, and the recent explosion of successful clinical trials-leading to the licensing of novel antibody-based therapeutic agents-has clearly demonstrated the power of recombinant antibody engineering, and greatly rejuvenated interest in their emergent clinical applications. In Diagnostic and Therapeutic Antibodies, a team of experts comprehensively reviews the theoretical and practical aspects of applying antibodies in both the laboratory and clinic. For general understanding, the book thoroughly introduces the basic science of the antibody molecule, including its structure and generation. Several novel in vivo applications of therapeutic antibodies are then presented to illustrate their special value in varied clinical settings. For those new to the field, there are readily reproducible methods for generating and purifying antibodies and for modifying them for clinical application, including how to measure their affinity and immunoactivity, and how to employ them in staining procedures and in a variety of in vitro assays. Timely and comprehensive, Diagnostic and Therapeutic Antibodies offers today,s clinical researchers a concise introduction to the field, as well as a highly useful compendium of practical protocols that will greatly facilitate the application of these powerful new diagnostic and therapeutic agents.
988			_aSpringer_Protocols_2000
650		7	_2embne _9405730 _aAnticuerpos monoclonales _xDiagnóstico
776	0	8	_iPrinted edition: _z9781617371950
776	0	8	_iPrinted edition: _z9780896037984
776	0	8	_iPrinted edition: _z9781489941473
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592590764 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b11/2023 _dz _ean _zSI