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008			100301s2004 xxu| fo |||| 0|eng d
020			_a9781592597741
024	7		_a10.1385/1592597742 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH443 _b2004 EB
245	0	0	_aRecombinant Gene Expression _bReviews and Protocols _cedited by Paulina Balbas, Argelia Lorence
250			_a2nd edition 2004
264		1	_aTotowa, NJ _bHumana Press _c2004
300			_a1 recurso en línea (XVI, 508 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v267
505	0		_aGeneral Issues About Recombinant Gene Expression -- Host Cell Compatibility in Protein Expression -- Production of Recombinant Proteins -- Folding-Promoting Agents in Recombinant Protein Production -- Prokaryotes -- Back to Basics -- ?-Complementation-Enabled T7 Expression Vectors and Their Use for the Expression of Recombinant Polypeptides for Protein Transduction Experiments -- Expression of Recombinant Alkaline Phosphatase Conjugates in Escherichia coli -- Overexpression of Chromosomal Genes in Escherichia coli -- Chromosomal Expression of Foreign and Native Genes From Regulatable Promoters in Escherichia coli -- Plasmid Vectors for Marker-Free Chromosomal Insertion of Genetic Material in Escherichia coli -- Copy-Control pBAC/oriV Vectors for Genomic Cloning -- Copy-Control Tightly Regulated Expression Vectors Based on pBAC/oriV -- Cell-Free Protein Synthesis With Prokaryotic Combined Transcription-Translation -- Genetic Tools for the Manipulation of Moderately Halophilic Bacteria of the Family Halomonadaceae -- Gene Transfer and Expression of Recombinant Proteins in Moderately Halophilic Bacteria -- Recombinant Protein Production in Antarctic Gram-Negative Bacteria -- Fungi -- Recombinant Protein Production in Yeasts -- Controlled Expression of Homologous Genes by Genomic Promoter Replacement in the Yeast Saccharomyces cerevisiae -- High-Throughput Expression in Microplate Format in Saccharomyces cerevisiae -- High-Throughput Expression in Microplate Format in Pichia pastoris -- Multiple Gene Expression by Chromosomal Integration and CRE-loxP-Mediated Marker Recycling in Saccharomyces cerevisiae -- Three Decades of Fungal Transformation -- Three Decades of Fungal Transformation -- Plants and Plant Cells -- Gene Transfer and Expression in Plants -- Production of Recombinant Proteins by Hairy Roots Cultured in Plastic Sleeve Bioreactors -- Engineering the Chloroplast Genome for Hyperexpression of Human Therapeutic Proteins and Vaccine Antigens -- New Selection Marker for Plant Transformation -- Enhancer Detection and Gene Trapping as Tools for Functional Genomics in Plants -- Animals and Animal Cells -- Gene Transfer and Expression in Mammalian Cell Lines and Transgenic Animals -- Sustained Heterologous Transgene Expression in Mammalian and Avian Cell Lines -- Inducible Gene Expression in Mammalian Cells and Mice -- Flp-Mediated Integration of Expression Cassettes into FRT-Tagged Chromosomal Loci in Mammalian Cells -- Generation of High-Recombinant-Protein-Producing Chinese Hamster Ovary (CHO) Cells -- Preparation of Recombinant Proteins in Milk.
520			_aIn this updated and expanded second edition of an established classic, the editors have added critical reviews to a fresh collection of cutting-edge protocols for gene expression in bacteria, fungi, plants, plant cells, animals, and animal cells. The review articles survey new directions in recombinant gene expression research, technique, and application, and point the way to using recombinant gene expression for metabolic engineering and the production of nonprotein molecules. The readily reproducible protocols offer new host-vector systems, such as Antarctic bacteria and moderately halophytic bacteria, vectors for chromosomal editing and metabolic engineering, and information to aid in problem solving, strategy planning, and the promotion of protein folding. There are also novel heterologous gene expression schemes for cell-free systems, plant chloroplasts, microplates, hairy roots, and animal milk, and new developments in consolidated heterologous systems. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and forward looking, Recombinant Gene Expression: Reviews and Protocols, Second Edition offers investigators seeking an overview of this critically important field not only the understanding, but also the tools needed to begin producing nonprotein products and altering the central metabolic pathways of cells to enhance heterologous gene expression.
988			_aSpringer_Protocols_2004
650		7	_2embne _9162770 _aExpresión génica
776	0	8	_iPrinted edition: _z9781617374609
776	0	8	_iPrinted edition: _z9781588292629
776	0	8	_iPrinted edition: _z9781489939265
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592597742 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b11/2023 _dz _ean _zSI