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003			ES-MaUEC
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007			cr nn 008mamaa
008			170831s2017 xxu| fo |||| 0|eng d
020			_a9781493972449
024	7		_a10.1007/978-1-4939-7244-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR502 _b2017 EB
245	0	0	_aPrions _bMethods and Protocols _cedited by Victoria A. Lawson
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XI, 371 páginas) _b52 ilustraciones, 26 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1658
505	0		_aPurification and Fibrillation of Full-length Recombinant PrP -- Method for Folding of Recombinant Prion Protein to Soluble b-sheet Secondary Structure -- Analysis of Prion Protein Conformation using Circular Dichroism Spectroscopy -- Analysis of Prion Protein Structure using Nuclear Magnetic Resonance Spectroscopy -- Immunodetection of PrPSc using Western Immunoblotting Techniques -- Analysis of miRNA Signatures in Neurodegenerative Prion Disease -- Expression of Heterologous PrP and Prion Propagation in RK13 Cells -- Generation of Infectious Prions and Detection with the Prion Infected Cell Assay -- Analysis of Cellular Prion Protein Endoproteolytic Processing -- Cellular Analysis Adult Neural Stem Cells for Investigating Prion Biology -- Neurotoxicity of Prion Peptides on Cultured Cerebellar Neurons -- Methods of Protein Misfolding Cyclic Amplification -- RT-QuIC Assays for Prion Disease Detection and Diagnostics -- A Quick Method to Evaluate the Effect of the Amino Acid Sequence in the Misfolding Proneness of the Prion Protein -- Insights into Mechanisms of Transmission and Pathogenesis from Transgenic Mouse Models of Prion Diseases.- In vivo Near Infrared Imaging of Neurodegeneration -- Strain Typing of Prion Diseases using in vivo Mouse Models -- Preparation and Immunostaining of the Myenteric Plexus of Prion Infected Mice -- Cell Culture Methods for Screening of Prion Therapeutics -- Real-Time Quaking Induced Conversion for Diagnosis of Prion Disease -- Methods for Molecular Diagnosis of Human Prion Disease -- Molecular sub-typing of PrPres in Human Sporadic CJD Brain Tissue -- Intercellular Prion-like Conversion and Transmission of Cu/Zn Superoxide Dismutase (SOD1) in Cell Culture.
520			_aThis volume details protocols on prion disease from multiple disciplines and highlights the contribution each discipline has made to the understanding of prion disease. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.     Authoritative and practical, Prions: Methods and Protocols aims to ensure successful results in the further study of this vital field.
988			_aSpringer_Protocols_2017
650		7	_2embne _9413113 _aVirología
776	0	8	_iPrinted edition: _z9781493972425
776	0	8	_iPrinted edition: _z9781493972432
776	0	8	_iPrinted edition: _z9781493984282
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7244-9 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b11/2023 _dz _ean _zSI