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008			110414s2009 xxu| fo |||| 0|eng d
020			_a9781607611035
024	7		_a10.1007/978-1-60761-103-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH605.3 _b2009 EB
245	0	0	_aMeiosis _bVolume 2, Cytological Methods _cedited by Scott Keeney
250			_a1st edition 2009
264		1	_aTotowa, NJ _bHumana Press _c2009
300			_a1 recurso en línea (X, 438 páginas) _b75 ilustraciones, 1 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v558
505	0		_aCytological Analysis of Meiotic Chromosomes in Fungi -- Chromosome Spreading and Immunofluorescence Methods in Saccharomyes cerevisiae -- Analysis of Schizosaccharomyces pombe Meiosis by Nuclear Spreading -- Assaying Chromosome Pairing by FISH Analysis of Spread Saccharomyces cerevisiae Nuclei -- Live-Cell Fluorescence Imaging of Meiotic Chromosome Dynamics in Schizosaccharomyces pombe -- Time-Lapse Fluorescence Microscopy of Saccharomyces cerevisiae in Meiosis -- Real-Time Imaging of Meiotic Chromosomes in Saccharomyces cerevisiae -- Observing Meiosis in Filamentous Fungi: Sordaria and Neurospora -- Meiotic Cytogenetics in Coprinus cinereus -- Cytological Analysis of Meiotic Chromosomes in Plants and Small Animals -- Cytological Analysis of Arabidopsis thaliana Meiotic Chromosomes -- Electron Microscopic Immunogold Localization of Recombination-Related Proteins in Spreads of Synaptonemal Complexes From Tomato Microsporocytes -- Cytological Analysis of Meiosis in Caenorhabditis elegans -- Cytological Analysis of Meiosis in Fixed Drosophila Ovaries -- Analysis of Chromosome Dynamics and Chromosomal Proteins in Drosophila Spermatocytes -- Methods for Meiotic Chromosome Preparation, Immunofluorescence, and Fluorescence in situ Hybridization in Daphnia pulex -- Immunofluorescent Microscopic Study of Meiosis in Zebrafish -- Cytological and Histological Analysis of Mammalian Germ Cells and Meiotic Chromosomes -- Staging of Mouse Seminiferous Tubule Cross-Sections -- Isolation and Short-Term Culture of Mouse Spermatocytes for Analysis of Meiosis -- Isolation and Analyses of Enriched Populations of Male Mouse Germ Cells by Sedimentation Velocity: The Centrifugal Elutriation -- Electron Microscopy of Mammalian Chromosomes -- Analyzing Mammalian Female Meiosis -- Cytological Analysis of Interference in Mouse Meiosis -- Analysis of Telomere Dynamics in Mouse Spermatogenesis -- Immunofluorescence Analysis of Human Spermatocytes -- Cytological Techniques to Study Human Female Meiotic Prophase -- Using RNA FISH to Study Gene Expression During Mammalian Meiosis.
520			_aThe unique chromosome dynamics of meiosis have fascinated scientists for well over a century, but in recent years there has been a proliferation of new data concerning the pairing, recombination, and segregation of chromosomes. In Meiosis, expert researchers explore recent advances in three main areas: quantitative genetic methods for analysis of homologous recombination and chromosome pairing, development of direct physical assays for DNA intermediates and products of recombination, and cytological methods for characterizing chromosome behaviors and the patterns by which specific proteins associate with meiotic chromosomes. Meiosis: Volume 2, Cytological Methods, details methods for preparing and examining meiotic tissues, cells, and chromosomes by electron microscopy, indirect immunofluorescence, fluorescent in situ hybridization, and live-cell imaging. The methods in this volume are grouped for convenience by general types of organisms: fungi, plants and small animals (mostly invertebrates), and mammals. Composed in the highly successful Methods in Molecular Biology(tm) series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Meiosis: Volume 2, Cytological Methods is an essential guidebook, providing up-to-date and critical new protocols for the study of meiotic chromosome dynamics.
988			_aSpringer_Protocols_2009
650		7	_2embne _9139477 _aCitología
776	0	8	_iPrinted edition: _z9781607611059
776	0	8	_iPrinted edition: _z9781607611028
776	0	8	_iPrinted edition: _z9781493958351
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60761-103-5 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b11/2023 _dz _ean _zSI