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008			100301s2007 xxu| fo |||| 0|eng d
020			_a9781597453882
024	7		_a10.1007/978-1-59745-388-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH442 _b2007 EB
245	0	0	_aIn Vitro Transcription and Translation Protocols _cedited by Guido Grandi
250			_a2nd edition 2007
264		1	_aTotowa, NJ _bHumana Press _c2007
300			_a1 recurso en línea (XII, 276 páginas) _b51 ilustraciones, 1 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v375
505	0		_aTechnologies -- Energy Systems for ATP Regeneration in Cell-Free Protein Synthesis Reactions -- Continuous-Exchange Protein-Synthesizing Systems -- Cell-Free Production of Integral Membrane Proteins on a Preparative Scale -- SIMPLEX: Single-Molecule PCR-Linked In Vitro Expression -- Methods for High-Throughput Materialization of Genetic Information Based on Wheat Germ Cell-Free Expression System -- Exogenous Protein Expression in Xenopus Oocytes -- Applications -- In Vitro Translation to Study HIV Protease Activity -- The Protein Truncation Test in Mutation Detection and Molecular Diagnosis -- Creation of Novel Enantioselective Lipases by SIMPLEX -- In Vitro Transcription and Translation Coupled to Two-Dimensional Electrophoresis for Bacterial Proteome Analysis -- In Vitro Screen of Bioinformatically Selected Bacillus anthracis Vaccine Candidates by Coupled Transcription, Translation, and Immunoprecipitation Analysis -- Functional Expression of Type 1 Rat GABA Transporter in Microinjected Xenopus laevis Oocytes -- Production of Protein for Nuclear Magnetic Resonance Study Using the Wheat Germ Cell-Free System.
520			_aA highly anticipated update of the previous edition, In Vitro Transcription and Translation Protocols, Second Edition, provides molecular biology laboratories with the most powerful techniques for exploiting in vitro transcription and translation systems.In Section One: Technologies, authors discuss the use of alternative energy systems for ATP regeneration, the efficient in vitro production of integral membrane proteins, and the high-throughput production of protein libraries using the innovative single-molecule PRC-linked in vitro expression (SIMPLEX) technology. In Section Two: Applications, authors present in vitro translation of amplified protease gene from HIV patients to select proper anti-protease drug therapy, in vitro translation of genes from human patients to diagnose biologically relevant gene mutations, use of SIMPLEX technology to select enantioselective lipases from libraries of randomly produced lipase mutants, and the in vitro transcription and translation to identify proteins in 2D-maps.
988			_aSpringer_Protocols_2007
650		7	_2embne _9147235 _aTranscripción genética
776	0	8	_iPrinted edition: _z9781617376412
776	0	8	_iPrinted edition: _z9781588295583
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-388-2 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b11/2023 _dz _ean _zSI