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008			160524s2016 xxu| fo |||| 0|eng d
020			_a9781493935666
024	7		_a10.1007/978-1-4939-3566-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP552 .C34 _b2016 EB
245	0	0	_aNLR Proteins _bMethods and Protocols _cedited by Francesco Di Virgilio, Pablo Pelegrín
250			_a1st edition 2016
264		1	_aNew York, NY _bSpringer International Publishing _c2016
300			_a1 recurso en línea (XI, 256 páginas) _b52 ilustraciones, 43 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1417
505	0		_aInnate Immune Receptors -- Atypical Inflammasomes -- Assessment of Inflammasome Activation By Cytokine And Danger Signal Detection -- Investigating IL-1β Secretion Using Real-Time Single-Cell Imaging -- Measuring IL-1β Processing By Bioluminescence Sensors I: Using a Bioluminescence Resonance Energy Transfer Biosensor -- Measuring IL-1β Processing By Bioluminescence Sensors II: The Igluc System -- Assessing Extracellular ATP as Danger Signal In Vivo: The Pmeluc System -- Measuring NLR Oligomerization I: Size Exclusion Chromatography, Co-Immunoprecipitation and Cross-Linking -- Measuring NLR Oligomerization II: Detection of ASC Speck Formation by Confocal Microscopy and Immunofluorescence -- Measuring NLR Oligomerization III: Detection of NLRP3 Complex by Bioluminescence Resonance Energy Transfer -- Measuring NLR Oligomerization IV: Using Förster Resonance Energy Transfer (FRET)-Fluorescence Lifetime Imaging Microscopy (FLIM) to Determine the Close Proximity Of Inflammasome Components -- Measuring NLR Oligomerization V: In Situ Proximity Ligation Assay -- Assessing Caspase-1 Activation -- Cell-Free Assay for Inflammasome Activation -- Functional Reconstruction of Nlrs In HEK293 Cells -- Method to Measure Ubiquitinization of Nlrs -- Cytofluorometric Quantification of Cell Death Elicited By NLR Proteins -- NLR in Human Diseases: Role and Laboratory Findings.
520			_aThis volume provides a sound basis for the molecular investigation of NLR function in health and disease. Chapters focus on of innate immune receptors, "atypical" inflammasomes, biochemical and novel bioluminescence techniques for the measurement of IL-1b, bioluminescent probe, biochemical and microscopy techniques, techniques to measure caspase-1 activation, cell free systems for the study of inflammasome function, and inflammasome activation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, NLR Proteins: Methods and Protocols aims to ensure successful results in the further study of this vital field.
988			_aSpringer_Protocols_2016
650		7	_2embne _9242917 _aProteínas
776	0	8	_iPrinted edition: _z9781493935642
776	0	8	_iPrinted edition: _z9781493935659
776	0	8	_iPrinted edition: _z9781493980796
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-3566-6 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b11/2023 _dz _ean _zSI