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008			111219s2012 xxu| fo |||| 0|eng d
020			_a9781617794773
024	7		_a10.1007/978-1-61779-477-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH599 _b2012 EB
245	0	0	_aChromatin Remodeling _bMethods and Protocols _cedited by Randall H. Morse
250			_a1st edition 2012
264		1	_aTotowa, NJ _bHumana Press _c2012
300			_a1 recurso en línea (XII, 453 páginas) _b83 ilustraciones, 5 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v833
505	0		_aStrain Construction and Screening Methods for a Yeast Histone H3/H4 Mutant Library -- Measuring Dynamic Changes in Histone Modifications and Nucleosome Density during Activated Transcription in Budding Yeast -- Monitoring the Effects of Chromatin Remodelers on Long-Range Interactions In Vivo -- Measuring Nucleosome Occupancy In Vivo by Micrococcal Nuclease -- Analysis of Nucleosome Positioning Using a Nucleosome-Scanning Assay (NuSA) -- Assaying Chromatin Structure and Remodeling by Restriction Enzyme Accessibility -- Generation of DNA Circles in Yeast by Inducible Site-Specific Recombination -- An Efficient Purification System for Native Minichromosome from S. cerevisiae -- Simultaneous, Single-Molecule Detection of Endogenous C-5 DNA Methylation and Chromatin Accessibility Using MAPit -- Analysis of Stable and Transient Protein-Protein Interactions -- Monitoring Dynamic Binding of Chromatin Proteins In Vivo by Fluorescence Recovery after Photobleaching (FRAP) -- Monitoring Dynamic Binding of Chromatin Proteins In Vivo by Fluorescence Correlation Spectroscopy (FCS) and Temporal Image Correlation Spectroscopy (TICS) -- Analysis of Chromatin Structure in Plant Cells -- Analysis of Histones and Histone Variants in Plants -- Reconstitution of Modified Chromatin Templates for In Vitro Functional Assays -- A Defined In Vitro System to Study ATP-Dependent Remodeling of Short Chromatin Fibers -- In Vitro Reconstitution of In Vivo-Like Nucleosome Positioning on Yeast DNA -- Activator-Dependent Acetylation of Chromatin Model Systems -- Mapping Assembly-Favored and Remodeled Nucleosome Positions on Polynucleosomal Templates -- Analysis of Changes in Nucleosome Conformation Using Fluorescence Resonance Energy Transfer (FRET) -- Preparation of Nucleosomes Containing a Specific H2A-H2A Crosslink Forming a DNA-Constraining Loop Structure -- Sulfyhydryl Reactive Site-Directed Crosslinking as a Method for Probing the Tetrameric Structure of Histones H3 and H4 -- Genomic Approaches for Determining Nucleosome Occupancy in Yeast -- Genome-Wide Approaches to Determining Nucleosome Occupancy in Metazoans Using MNase-Seq -- Salt Fractionation of Nucleosomes for Genome-Wide Profiling -- Quantitative Analysis of Genome-Wide Chromatin Remodeling -- Computational Analysis of Nucleosome Positioning.
520			_aChromatin is of central importance to gene regulation in eukaryotes.  Reflecting this singular role for chromatin, numerous approaches have evolved in the laboratory over the past three decades to study chromatin structure and its alterations. Methods of investigating chromatin remodeling, whether in changes in nucleosome structure or position with respect to the incorporated DNA or in histone modifications, have progressed rapidly over the recent years.  In Chromatin Remodeling: Methods and Protocols, expert researchers contribute chapters which include methods for investigating chromatin remodeling in vitro and in vivo, in yeast, plants, and mammalian cells, and at local and global levels.  Both gene-specific and genome-wide approaches are covered, and in recognition of the increasing prevalence of the latter type of study, the final two chapters focus on bioinformatic/computational approaches to analyzing genome-wide data on chromatin structure. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Comprehensive and essential, Chromatin Remodeling: Methods and Protocols serves as a key source of clear techniques as well as a collection of chapters that can inspire future techniques in this vital field of study.
988			_aSpringer_Protocols_2012
650		7	_2embne _9144121 _aCromatina
776	0	8	_iPrinted edition: _z9781617794766
776	0	8	_iPrinted edition: _z9781617794780
776	0	8	_iPrinted edition: _z9781493962136
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-477-3 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b11/2023 _dz _ean _zSI