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020 _a9781493927487
024 7 _a10.1007/978-1-4939-2748-7
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQK617.5
_b2015 EB
245 0 0 _aYeast Surface Display
_bMethods, Protocols, and Applications
_cedited by Bin Liu
250 _a1st edition 2015
264 1 _aNew York, NY
_bSpringer International Publishing
_c2015
300 _a1 recurso en línea (XI, 271 páginas)
_b41 ilustraciones, 28 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1319
505 0 _aProtein Engineering and Selection Using Yeast Surface Display -- Isolation and Validation of Anti-B7-H4 scFvs from an Ovarian Cancer scFv yeast-Display Library -- Combining Phage and Yeast Cell Surface Antibody Display to Identify Novel Cell Type-Selective Internalizing Human Monoclonal Antibodies -- Yeast Display-Based Antibody Affinity Maturation Using Detergent-Solubilized Cell Lysates -- Yeast Endoplasmic Reticulum Sequestration Screening for the Engineering of Proteases from Libraries Expressed in Yeast -- T Cell Receptor Engineering and Analysis Using the Yeast Display Platform -- Epitope Specific Binder Design by Yeast Surface Display -- Applications of Yeast Surface Display for Protein Engineering -- Identification of Novel Protein-Ligand Interactions by Exon Microarray Analysis of Yeast Surface Displayed cDNA Library Selection Outputs -- Identification of Post-Translational Modification-Dependent Protein Interactions using Yeast Surface Displayed Human Proteome Libraries -- Utilizing Yeast Surface Human Proteome Display Libraries to Identify Small Molecule-Protein Interactions -- Enzyme Evolution by Yeast Cell Surface Engineering -- Electrochemical Glucose Biosensor Based on Glucose Oxidase Displayed on Yeast Surface -- Couple Binding to Catalysis - Use Yeast Cell Surface Display to Select Enzymatic Activities -- The use of Yeast Surface Display in Biofuel Cells.
520 _aIn addition to research and discovery, yeast surface display technology has found applications in industrial processes such as biofuel production and environmental pollutant absorption and degradation. Yeast Surface Display: Methods, Protocols, and Applications guides readers through yeast surface antibody display library and antibody engineering,  yeast surface display as a tool for protein engineering, yeast surface cDNA display library construction and applications, and yeast surface display in bioassay and industrial applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Concise and easy-to-use, Yeast Surface Display: Methods, Protocols, and Applications aims to help accelerate the work of protein chemists, antibody engineers, molecular and cell biologists, and industrial bioengineers.
988 _aSpringer_Protocols_2015
650 7 _2embne
_9138683
_aHongos
776 0 8 _iPrinted edition:
_z9781493927470
776 0 8 _iPrinted edition:
_z9781493927494
776 0 8 _iPrinted edition:
_z9781493946679
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-2748-7
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b11/2023
_dz
_ean
_zSI