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008			100301s2006 xxu| fo |||| 0|eng d
020			_a9781592599592
024	7		_a10.1385/1592599591 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQK725 _b2006 EB
245	0	0	_aPlant Cell Culture Protocols _cedited by Víctor M. Loyola-Vargas, Felipe Vázquez-Flota
250			_a1st edition 2006
264		1	_aTotowa, NJ _bHumana Press _c2006
300			_a1 recurso en línea (XVI, 394 páginas) _b77 ilustraciones, 3 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v318
505	0		_aAn Introduction to Plant Cell Culture -- History of Plant Tissue Culture -- Cell Culture and Plant Regeneration: The Fundamentals -- Pathogen and Biological Contamination Management -- Growth Measurements -- Callus and Suspension Culture Induction, Maintenance, and Characterization -- Measurement of Cell Viability in In Vitro Cultures -- Cryopreservation of Embryogenic Cell Suspensions by Encapsulation-Vitrification -- Somatic Embryogenesis in Picea Suspension Cultures -- Indirect Somatic Embryogenesis in Cassava for Genetic Modification Purposes -- Direct Somatic Embryogenesis in Coffea canephora -- PLANT PROPAGATION IN VITRO -- A New Temporary Immersion Bioreactor System for Micropropagation -- Protocol to Achieve Photoautotrophic Coconut Plants Cultured In Vitro With Improved Performance Ex Vitro -- Use of Statistics in Plant Biotechnology -- An Efficient Method for the Micropropagation of Agave Species -- Micropropagation of Endangered Plant Species -- Clonal Propagation of Softwoods -- Applications for Plant Protoplasts -- Isolation, Culture, and Plant Regeneration From Leaf Protoplasts of Passiflora -- Isolation, Culture, and Plant Regeneration From Echinacea purpurea Protoplasts -- Production of Cybrids in Brassicaceae Species -- Guard Cell Protoplasts -- Production of Interspecific Hybrid Plants in Primula -- Protocols for Genomic Manipulation -- Agrobacterium-Mediated Transformation of Petunia Leaf Discs -- Transformation of Wheat Via Particle Bombardment -- Chloroplast Transformation -- The Biochemical Basis for the Resistance to Aluminum and Their Potential as Selection Markers -- Transformation of Maize Via Agrobacterium tumefaciens Using a Binary Co-Integrate Vector System -- Accumulation of Metabolites in Plant Cells -- Capsaicin Accumulation in Capsicum spp. Suspension Cultures -- Isolation and Purification of Ribosome-Inactivating Proteins -- Catharanthus roseus Shoot Cultures for the Production of Monoterpenoid Indole Alkaloids -- Methods for Regeneration and Transformation in Eschscholzia californica.
520			_aFollowing in the footsteps of its much-acclaimed first edition, Plant Cell Culture Protocols, Second Edition has been expanded and revised to include the most up-to-date and frequently used techniques for plant cell and tissue culture. Readily reproducible and extensively annotated, the methods range from general methodologies, such as culture induction, growth and viability evaluation, and contamination control, to such highly specialized techniques as chloroplast transformation involving the laborious process of protoplast isolation and culture. Most of the protocols are currently used in the research programs of the authors or represent important parts of business projects aimed at the generation of improved plant materials. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Two new appendices have also been added to explain the principles for formulating culture media and the composition of the eight most commonly used media formulations, as well as to list more than 100 very useful internet sites. Comprehensive and highly practical, Plant Cell Culture Protocols, Second Edition offers today's plant biotechnologists indispensable state-of-the-art techniques for carrying out plant research, for developing new transgenic plants, and for today's many and diverse commercial applications.
988			_aSpringer_Protocols_2006
650		7	_2embne _9138491 _aPlantas _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781617376344
776	0	8	_iPrinted edition: _z9781588295477
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592599591 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b11/2023 _dz _ean _zSI