000			04323nam a22003975i 4500
999			_c392801 _d392801
001			392801
003			ES-MaUEC
005			20240302163318.0
006			a||||fo|||| 00| 0
007			cr nn 008mamaa
008			131028s2014 xxu| fo |||| 0|eng d
020			_a9781627037181
024	7		_a10.1007/978-1-62703-718-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH641 _b2014 EB
245	0	0	_aBioluminescent Imaging _bMethods and Protocols _cedited by Christian E. Badr
250			_a1st edition 2014
264		1	_aTotowa, NJ _bHumana Press _c2014
300			_a1 recurso en línea (XI, 276 páginas) _b50 ilustraciones, 39 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1098
505	0		_aBioluminescence Imaging: Basics and Practical Limitations -- Extraction and Quantification of Adenosine Triphosphate in Mammalian Tissues and Cells -- Neuronal Network Imaging in Acute Slices Using Ca2+ Sensitive Bioluminescent Reporter -- Gaussia Luciferase-Based Mycoplasma Detection Assay in Mammalian Cell Culture -- Split Gaussia Luciferase for Imaging Ligand-Receptor Binding -- Video-Rate Bioluminescence Imaging of Protein Secretion from a Living Cell -- Bioluminescence Reporter Gene-Based Detection of MicroRNAs -- Monitoring of Transcriptional Dynamics of HIF and NFκB Activities -- Real-Time Bioluminescent Tracking of Cellular Population Dynamics -- Fabrication of Bioluminescent Capsules and Live-Cell Imaging -- The Bioluminescent Imaging of Spontaneously Occurring Tumors in Immunocompetent ODD-Luciferase Bearing Transgenic Mice -- Blood-Based Assay with Secreted Gaussia Luciferase to Monitor Tumor Metastasis -- Bioluminescence Imaging of Fungal Biofilm Development in Live Animals -- Bioluminescent Imaging of Bacteria During Mouse Infection -- Cell-Based Bioluminescence Screening Assays -- Bioluminescence-Based Monitoring of Virus Vector-Mediated Gene Transfer in Mice -- Simultaneous In Vivo Monitoring of Regulatory and Effector T Lymphocytes Using Secreted Gaussia Luciferase, Firefly Luciferase, and Secreted Alkaline Phosphatase -- Multiplex Functional Bioluminescent Reporters Using Gaussia Luciferase Fused to Epitope Tags in an Immunobinding Assay -- Non-Invasive In Vivo Monitoring of Extracellular Vesicles -- In Vitro and In Vivo Demonstrations of Fluorescence by Unbound Excitation from Luminescence (FUEL).
520			_aBioluminescent Imaging: Methods and Protocols distills a wide range of techniques that use bioluminescence imaging as a tool for visualizing and tracking various biological processes. Covering diverse fields such as cellular and molecular biology, oncology, neurology, infectious diseases, immunology, and others, the detailed chapters of this volume are arranged by topic and describe practical procedures and applications of different bioluminescent reporters, from photoproteins (Aequorin) to bacterial luciferases as well as other secreted (such as Gaussia) and non-secreted luciferases (such as Firefly).  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips for troubleshooting and avoiding known pitfalls.   Authoritative and cutting-edge, Bioluminescent Imaging: Methods and Protocols aims to provide diverse and comprehensive techniques to researchers interested in implementing bioluminescence-based imaging in their laboratory, regardless of their previous level of experience with such methodologies.
988			_aSpringer_Protocols_2014
650		7	_2embne _9139477 _aCitología
776	0	8	_iPrinted edition: _z9781627037198
776	0	8	_iPrinted edition: _z9781627037174
776	0	8	_iPrinted edition: _z9781493960675
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-718-1 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b11/2023 _dz _ean _zSI