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008			161115s2017 gw | fo |||| 0|eng d
020			_a9783662491409
024	7		_a10.1007/978-3-662-49140-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP751 _b2017 EB
245	0	0	_aHydrocarbon and Lipid Microbiology Protocols _bActivities and Phenotypes _cedited by Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales
250			_a1st edition 2017
264		1	_aBerlin, Heidelberg _bSpringer International Publising _c2017
300			_a1 recurso en línea (X, 244 páginas) _b40 ilustraciones, 20 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aSpringer Protocols Handbooks _x1949-2456
505	0		_aIntroduction -- Protocols for the measurement of bacterial chemotaxis to hydrocarbons -- Determining the tendency of microorganisms to interact with hydrocarbon phases -- Protocols for measuring biosurfactant production in microbial cultures -- Protocol for the measurement of hydrocarbon transport in bacteria -- Bacterial solvent response and tolerance: cis-trans isomerization -- Analysis of PHB metabolism applying Tn5 mutagenesis in Ralstonia eutropha -- Phenotyping microarrays for the characterization of environmental microorganisms -- Control of the concentration of aquatic hydrocarbons by microorganisms -- Protocols for measuring methanogenesis -- Respiration rate determined by phosphorescence-based sensors -- Measuring the impact of hydrocarbons on rates of nitrogen fixation -- Protocols for measuring activity of sulphate-reducing bacteria in water injection systems -- Protocol for evaluating the biological stability of fuel formulations and their relationship to carbon steel biocorrosion -- Laboratory protocols for investigating microbial souring and potential treatments in crude oil reservoirs.
520			_aThis Volume features protocols for investigating the hydrocarbon- and lipid-specific activities of microbes. They include methods for studying chemotaxis, the colonisation of hydrocarbon surfaces, hydrocarbon uptake, respiration, nitrogen fixation, sulphate reduction, membrane stabilisation through cis-trans isomerisation of membrane fatty acids, and the production of biosurfactants and biopolymers in response to the presence of hydrocarbons. A protocol for studying the ability of microbes to control the concentration of hydrocarbons in their aqueous environment is also described, and phenotyping methods to reveal microbes' more general metabolic activities are presented. Several protocols for investigating acid production in connection with oil souring and biocorrosion by microbes in oil well, oil transportation and storage settings are presented. Lastly, protocols for measuring methanogenesis, as an example of microbial hydrocarbon production, are described. Hydrocarbon and Lipid Microbiology Protocols There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.
988			_aSpringer_Protocols_2017
650		7	_2embne _9142933 _aLípidos
776	0	8	_iPrinted edition: _z9783662491386
776	0	8	_iPrinted edition: _z9783662491393
776	0	8	_iPrinted edition: _z9783662569856
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-3-662-49140-9 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b11/2023 _dz _ean _zSI