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005			20231104181116.0
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007			cr nn 008mamaa
008			100301s2006 xxu| fo |||| 0|eng d
020			_a9781597451437
024	7		_a10.1385/1597451436 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR67.2 _b2006 EB
245	0	0	_aDiagnostic Bacteriology Protocols _cedited by Louise O'Connor
250			_a2nd edition 2006
264		1	_aTotowa, NJ _bHumana Press _c2006
300			_a1 recurso en línea (X, 226 páginas) _b51 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v345
505	0		_aA Review of Current and Future Molecular Diagnostic Tests for Use in the Microbiology Laboratory -- Overview of DNA Purification for Nucleic Acid-Based Diagnostics From Environmental and Clinical Samples -- Microarray-Based Detection of Bacteria by On-Chip PCR -- An Array Biosensor for Detection of Bacterial and Toxic Contaminants of Foods -- Detection of Neisseria meningitidis, Streptococcus pneumoniae, and Haemophilus influenzae in Blood and Cerebrospinal Fluid Using Fluorescence-Based PCR -- Use of Hybridization Probes in a Real-Time PCR Assay on the LightCycler® for the Detection of Methicillin-Resistant Staphylococcus aureus -- Detection of Verotoxin Genes VT 1 and VT 2 in Escherichia coli O157:H7 in Minced Beef Using Immunocapture and Real-Time PCR -- Application of Two-Step Quantitative Reverse-Transcription PCR to Bacterial Diagnostics -- Quencher Extension for Single Nucleotide Polymorphism Quantification in Bacterial Typing and Microbial Community Analyses -- Amplified Fragment-Length Polymorphism and Protein Profiling for Identification of Campylobacter lari Subgroups -- Use of Peptide Nucleic Acid Probes for Rapid Detection and Enumeration of Viable Bacteria in Recreational Waters and Beach Sand -- HOOF Prints -- Detection of Legionella in Various Sample Types Using Whole-Cell Fluorescent In Situ Hybridization -- Identification of Diagnostic Proteins in Mycobacterium avium subspecies paratuberculosis by a Whole Genome Analysis Approach -- Diagnosis of Q Fever Using Indirect Microimmunofluorescence -- Macrophage Cell Cultures for Rapid Isolation of Intracellular Bacteria.
520			_aCarrying on the high standards of the much respected first edition, Diagnostic Bacteriology Protocols, Second Edition, has been fully updated to include the novel identification, detection, and typing technologies that are transforming the field of diagnostic testing. The authors, working at the forefront of diagnostic test development, highlight these new technologies and describe current and future molecular diagnostic tests and related nucleic acid extraction methods. The protocols range from advanced molecular detection, quantification, and typing systems, to protocols for diagnostic protein identification, serological testing, and cell culture-based assays. The emphasis is on nucleic acid-based diagnostics and alternative biochemically and immunologically based formats that can provide significant potential for multiparameter testing and automation. Several protocols describe specific organisms that may be easily modified to detect other species of interest. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Forward looking and highly practical, Diagnostic Bacteriology Protocols, Second Edition illuminates the cutting-edge technologies now available for bacterial pathogen detection and identification and demonstrates their power and ease of use in a wide variety of applications.
988			_aSpringer_Protocols_2006
650		7	_2embne _9138684 _aMicrobiología
776	0	8	_iPrinted edition: _z9781617376665
776	0	8	_iPrinted edition: _z9781588295941
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1597451436 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b11/2023 _dz _ean _zSI