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020 _a9781493964789
024 7 _a10.1007/978-1-4939-6478-9
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aTP248.25 .B55
_b2016 EB
245 0 0 _aBioreactors in Stem Cell Biology
_bMethods and Protocols
_cedited by Kursad Turksen
250 _a1st edition 2016
264 1 _aNew York, NY
_bSpringer International Publishing
_c2016
300 _a1 recurso en línea (XIV, 238 páginas)
_b43 ilustraciones, 41 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1502
505 0 _aMulti-Compartmental Hollow-Fiber-Based Bioreactors for Dynamic Three-Dimensional Perfusion Culture -- A Bioreactor to Apply Multi-Modal Physical Stimuli to Cultured Cells -- Aggregate and Microcarrier Cultures of Human Pluripotent Stem Cells in Stirred Suspension Systems -- Expansion of Human Induced Pluripotent Stem Cells in Stirred Suspension Bioreactors -- Uniformed Embryoid Body Production and Enhanced Mesendoderm Differentiation with Murine Embryonic Stem Cells in a Rotary Suspension Bioreactor -- Expansion of Human Mesenchymal Stem Cells in a Microcarrier Bioreactor -- Large-Scale Expansion and Differentiation of Mesenchymal Stem Cells in Microcarrier-Based Stirred Bioreactors -- Bioreactor Expansion of Skin-Derived Precursor Schwann Cells -- Use of Stirred Suspension Bioreactors for Male Germ Cell Enrichment -- Generation of Neural Progenitor Spheres from Human Pluripotent Stem Cells in a Suspension Bioreactor -- Perfusion Stirred-Tank Bioreactors for 3D Differentiation of Human Neural Stem Cells -- Scalable Expansion of Human Pluripotent Stem Cell-Derived Neural Progenitors in Stirred Suspension Bioreactor Under Xeno-Free Condition -- A Microfluidic Bioreactor for Toxicity Testing of Stem Cell Derived 3D Cardiac Bodies -- Novel Bioreactor Platform for Scalable Cardiomyogenic Differentiation from Pluripotent Stem Cell-Derived Embryoid Bodies -- Whole Heart Construct Cultivation Under 3D Mechanical Stimulation of the Left Ventricle -- Tendon Differentiation on Decellularized Extracellular Matrix Under Cyclic Loading -- Bioengineered Models of Solid Human Tumors for Cancer Research -- Development of a Bladder Bioreactor for Tissue-Engineering in Urology -- Use of Microfluidic Technology to Monitor the Differentiation and Migration of Human ESC-Derived Neural Cells.
520 _aThis meticulous volume recognizes the need to translate what has been learned primarily in tissue culture dishes to approaches supporting scale-up studies, not only to large quantities of cells but also to heterogeneous cell constructs. Notable advances are being made in these latter approaches, prompting this collection of a variety of representative protocols that facilitate important modifications and novel approaches to bioreactors in stem cell research, contributed by both established and new investigators in this area. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Bioreactors in Stem Cell Biology: Methods and Protocols will serve as an ideal guide for scientists seeking to increase our understanding of stem cells and their potential to repair and regenerate tissues and organs.
988 _aSpringer_Protocols_2016
650 7 _2embne
_9140931
_aBiotecnología
776 0 8 _iPrinted edition:
_z9781493964765
776 0 8 _iPrinted edition:
_z9781493964772
776 0 8 _iPrinted edition:
_z9781493982134
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6478-9
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b10/2023
_dz
_ean
_zSI