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008			110414s2009 xxu| fo |||| 0|eng d
020			_a9781597455541
024	7		_a10.1007/978-1-59745-554-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aRM282 .I44 _b2009 EB
245	0	0	_aTherapeutic Antibodies _bMethods and Protocols _cedited by Antony S. Dimitrov
250			_a1st edition 2009
264		1	_aTotowa, NJ _bHumana Press _c2009
300			_a1 recurso en línea (XVIII, 585 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v525
505	0		_aRecombinant Antigens -- Therapeutic Antibodies: Current State and Future Trends - Is a Paradigm Change Coming Soon? -- Preparation of Recombinant Viral Glycoproteins for Novel and Therapeutic Antibody Discovery -- Antibody Libraries -- Design of a Human Synthetic Combinatorial Library of Single-Chain Antibodies -- Construction of a Human Antibody Domain (VH) Library -- Generation and Selection of Rabbit Antibody Libraries by Phage Display -- Construction of a Large Naïve Human Phage-Displayed Fab Library Through One-Step Cloning -- Antibody Discovery -- Identification of Target and Function Specific Antibodies for Effective Drug Delivery -- Screening of Specific Internalization Fab Fragment from Human Naive Phage Library by Combinational Bio-Panning -- Competitive Antigen Panning for Selection of HIV-1 Neutralizing Human Monoclonal Antibodies Specific for gp41 -- Selection of Non-aggregating VH Binders from Synthetic VH Phage-Display Libraries -- Isolation of Full-Length IgG Antibodies from Combinatorial Libraries Expressed in Escherichia coli -- Multiplexed Flow Cytometry: High-Throughput Screening of Single-Chain Antibodies -- Human Antibody Repertoires -- Rapid Screening Platform for Stabilization of scFvs in Escherichia coli -- Antibody Engineering -- In Vitro Antibody Affinity Maturation Targeting Germline Hotspots -- Affinity Maturation by Phage Display -- Production of Chimeric Heavy-Chain Antibodies -- Mammalian Cell Display for Antibody Engineering -- Improving Antibody Binding Affinity and Specificity for Therapeutic Development -- Construction and Production of an IgG-Like Tetravalent Bispecific Antibody for Enhanced Therapeutic Efficacy -- Deimmunization of Monoclonal Antibodies -- Anti-CD22 Onconase: Preparation and Characterization -- Antibody-Cytotoxic Agent Conjugates: Preparation and Characterization -- Antibody Preclinical Development -- High-Level Production of a Humanized ImmunoRNase Fusion Protein from Stably Transfected Myeloma Cells -- Antibody Fragment Expression and Purification -- Scaling-Up and Production of Therapeutic Antibodies for Preclinical Studies -- Antibody Production by the Gram-Positive Bacterium Bacillus megaterium -- Neutralization of HIV by Antibodies -- Dynamics of Antibody Domains Studied by Solution NMR -- In Vivo Method for Establishing Synergy Between Antibodies to Epidermal Growth Factor Receptor and Vascular Endothelial Growth Factor Receptor-2 -- Passive Immunization with Human Neutralizing Monoclonal Antibodies Against HIV-1 in Macaque Models: Experimental Approaches -- Antibody Sequence Analysis -- Organizing, Exploring, and Analyzing Antibody Sequence Data: The Case for Relational-Database Managers.
520			_aWith revenues from the top five therapeutic antibodies accounting for a majority of the recent pharmaceutical sales, the research and development in the field has exploded over the past several years and is expected to grow with new emerging monoclonal antibodies like Numax, Lucentis, Actemra, and others. In Therapeutic Antibodies: Methods and Protocols, leading experts from academic laboratories and biotechnology companies present an extensive set of protocols for the discovery and development of therapeutic antibodies, featuring sections devoted to recombinant antigens, antibody libraries, antibody discovery, antibody engineering, and antibody preclinical development. Written in the highly successful Methods in Molecular Biology™ series format, the chapters contain brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Therapeutic Antibodies: Methods and Protocols serves as a key resource for researchers and antibody engineers investigating and participating in this rapidly growing therapeutic market segment.
988			_aSpringer_Protocols_2009
650		7	_2embne _9143641 _aInmunoglobulinas
776	0	8	_iPrinted edition: _z9781603278256
776	0	8	_iPrinted edition: _z9781934115923
776	0	8	_iPrinted edition: _z9781627038690
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-554-1 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _dz _ean _zSI