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003			ES-MaUEC
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007			cr nn 008mamaa
008			100301s1996 xxu| fo |||| 0|eng d
020			_a9781592595440
024	7		_a10.1385/0896033325 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH465 .A1 _b1996 EB
245	0	0	_aIn Vitro Mutagenesis Protocols _cedited by Michael K. Trower
250			_a1st edition 1996
264		1	_aTotowa, NJ _bHumana Press _c1996
300			_a1 recurso en línea (XIV, 391 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v57
505	0		_aSite-Directed Mutagenesis Using Positive Antibiotic Selection -- In Vitro Site-Directed Mutagenesis Using the Unique Restriction Site Elimination (USE) Method -- Site-Directed Mutagenesis Using Double-Stranded Plasmid DNA Templates -- Site-Directed Mutagenesis Using a Uracil-Containing Phagemid Template -- Oligonucleotide-Directed Mutagenesis Using an Improved Phosphorothioate Approach -- Analysis of Point Mutations by Use of Amber Stop Codon Suppression -- A Simple Method for Site-Directed Mutagenesis with Double-Stranded Plasmid DNA -- Double-Stranded DNA Site-Directed Mutagenesis -- Solid-Phase In Vitro Mutagenesis Using a Plasmid DNA Template -- Targeted Mutagenesis Mediated by the Triple Helix Formation -- A Universal Nested Deletion Method Using an Arbitrary Primer and Elimination of a Unique Restriction Site -- Ordered Deletions Using Exonuclease III -- Ligase Chain Reaction for Site-Directed In Vitro Mutagenesis -- PCR-Based Site-Directed Mutagenesis -- In Vitro Recombination and Mutagenesis by Overlap Extension PCR -- Site-Directed Mutagenesis Using Overlap Extension PCR -- Modification of the Overlap Extension Method for Extensive Mutagenesis on the Same Template -- Site-Directed Mutagenesis In Vitro by Megaprimer PCR -- Using PCR for Rapid Site-Specific Mutagenesis in Large Plasmids -- PCR-Assisted Mutagenesis for Site-Directed Insertion/Deletion of Large DNA Segments -- Site-Directed Mutagenesis Using a Rapid PCR-Based Method -- A Simple Method to Introduce Internal Deletions or Mutations into Any Position of a Target DNA Sequence -- A Simple Method for Site-Specific Mutagenesis that Leaves the Rest of the Template Unaltered -- Multiple Site-Directed Mutagenesis -- Construction of Linker-Scanning Mutations by Oligonucleotide Ligation -- Construction of Linker-Scanning Mutations Using PCR -- Use of Codon Cassette Mutagenesis for Saturation Mutagenesis -- Saturation Mutagenesis by Mutagenic Oligonucleotide-Directed PCR Amplification (Mod-PCR) -- Random Mutagenesis of Short Target DNA Sequences via PCR with Degenerate Oligonucleotides -- Random Sequence Mutagenesis for the Generation of Active Enzymes -- Random Mutagenesis by Using Mixtures of dNTP and dITP in PCR -- PCR-Mediated Chemical Mutagenesis -- Oligonucleotide-Directed Random Mutagenesis Using the Phosphorothioate Method -- An Efficient Random Mutagenesis Technique Using an E. coli Mutator Strain.
988			_aSpringer_Protocols_1996
650		7	_2embne _9140806 _aADN
776	0	8	_iPrinted edition: _z9780896033320
776	0	8	_iPrinted edition: _z9781489940292
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896033325 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _dz _ean _zSI