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008			141013s2015 xxu| fo |||| 0|eng d
020			_a9781493916801
024	7		_a10.1007/978-1-4939-1680-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH595 _b2015 EB
245	0	4	_aThe Nucleus _cedited by Ronald Hancock
250			_a2nd edition 2015
264		1	_aNew York, NY _bSpringer International Publishing _c2015
300			_a1 recurso en línea (XI, 237 páginas) _b54 ilustraciones, 35 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1228
505	0		_aCell Type-Specific Affinity Purification of Nuclei for Chromatin Profiling in Whole Animals -- Lysis Gradient Centrifugation - A Flexible Method for the Isolation of Nuclei from Primary Cells -- Isolation of Nuclei in Media Containing an Inert Polymer to Mimic the Crowded Cytoplasm -- A New Rapid Method for Isolating Nucleoli -- Sequential Recovery of Macromolecular Components of the Nucleolus -- Au Nanoinjectors for Electrotriggered Gene Delivery into the Cell Nucleus -- Improving Chromatin Immunoprecipitation (ChIP) by Suppression of Method-Induced DNA-Damage Signaling -- Purification of Specific Chromatin Loci for Proteomic Analysis -- Chromatin Structure Analysis of Single Gene Molecules by Psoralen Crosslinking and Electron Microscopy -- Purification of Proteins on Newly Synthesized DNA Using iPOND -- Applying the Ribopuromycylation Method to Detect Nuclear Translation -- Targeted Nano Analysis of Water and Ions in the Nucleus Using Cryo-Correlative Microscopy -- A Redox-Sensitive Yellow Fluorescent Protein Sensor for Monitoring Nuclear Glutathione Redox  Dynamics -- Determination of the Dissociation Constant of the NFkB p50/p65 Heterodimer in Living Cells Using Fluorescence Cross-Correlation Spectroscopy -- Imaging and Quantification of Amyloid Fibrillation in the Cell Nucleus -- Analysis of Nuclear Organization with TANGO, Software for High-Throughput Quantitative Analysis of 3D Fluorescence Microscopy Images -- Quantitative Analysis of Chromosome Localization in the Nucleus.
520			_aThis volume presents detailed, recently-developed protocols ranging from isolation of nuclei to purification of chromatin regions containing single genes, with a particular focus on some less well-explored aspects of the nucleus. The methods described include new strategies for isolation of nuclei, for purification of cell type-specific nuclei from a mixture, and for rapid isolation and fractionation of nucleoli. For gene delivery into and expression in nuclei, a novel gentle approach using gold nanowires is presented. As the concentration and localization of water and ions are crucial for macromolecular interactions in the nucleus, a new approach to measure these parameters by correlative optical and cryo-electron microscopy is described. The Nucleus, Second Edition presents methods and software for high-throughput quantitative analysis of 3D fluorescence microscopy images, for quantification of the formation of amyloid fibrils in the nucleus, and for quantitative analysis of chromosome territory localization. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, The Nucleus, Second Edition seeks to serve both professionals and novices with its well-honed methods for the study of the nucleus.
988			_aSpringer_Protocols_2015
650		7	_2embne _9139477 _aCitología
776	0	8	_iPrinted edition: _z9781493916795
776	0	8	_iPrinted edition: _z9781493916818
776	0	8	_iPrinted edition: _z9781493948734
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-1680-1 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _dz _ean _zSI