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008			140206s1998 xxu| fo |||| 0|eng d
020			_a9781592592142
024	7		_a10.1385/0896033546 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH585.5 .F56 _b1998 EB
245	0	0	_aFlow Cytometry Protocols _cedited by Mark J. Jaroszeski, Richard Heller
250			_a1st edition 1998
264		1	_aTotowa, NJ _bHumana Press _c1998
300			_a1 recurso en línea (X, 274 páginas) _b56 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v91
505	0		_aBasics of Flow Cytometry -- Detection of Terminal Transferase in Leukemia -- The Use of Flow Cytometry to Detect Intracellular Cytokine Production in Individual Cells -- Detection of Intracellular/lntranuclear Antigens -- Detection of Intracellular/lntranuclear Antigens in Tumor Cells -- Detection of Cyclins in Individual Cells by Flow and Laser Scanning Cytometry -- Estimation of Cell Viability by Flow Cytometry -- Flow Cytometric Detection of Fluorescent Redistributional Dyes for Measurement of Cell Transmembrane Potential -- Flow Cytometric Analysis of Macrophage Oxidative Metabolism Using DCFH -- Measurement of Environmental Particulate Uptake by Lung Cells Using Flow Cytometry -- Estimation of Drug Resistance by Flow Cytometry -- Flow Cytometric Monitoring of Cellular Pharmacokinetics -- Quantification of Xenoprotein Electroinsertion in Mammalian Cells -- Flow Cytometry Quantification of Electropermeabilization -- Flow Cytometric Detection and Quantitation of Cell-Cell Electrofusion Products -- Cell Cycle Analysis of Asynchronous Populations -- Simultaneous DNA/RNA Analysis of Solid and Hematoreticular Malignancies -- Solid Tumor DNA Ploidy Analysis -- FISHing for Cytokines -- Analysis of Apoptosis by Flow Cytometry -- Chromosome Sorting and Analysis by FACS -- Simultaneous Five-Six Color Multiparameter Analysis.
520			_aIn Flow Cytometry Protocols, leading researchers and experimenters update the complex and technically sophisticated field of flow cytometry with detailed accounts of their many new, improved, and well-tested protocols and applications. Among the cutting-edge methods presented here are techniques for the detection of terminal transferase ion leukemia, intracellular cytokines, intracellular/intranuclear antigens in leukemia and tumors, and cyclins. Protocols for performing functional studies include methods for assessing viability, transmembrane potential, oxidative metabolism, environmental particulate uptake, drug resistance, and cellular pharmacokinetics. The methods for examining DNA address cell cycle analysis, DNA/RNA analysis of solid malignancies, solid-tumor DNA ploidy analysis, apoptosis, and a method that combines FISH with flow cytometry. Advanced methods for chromosome sorting and 5-6 color analysis are also detailed. Flow Cytometry Protocols contains methods that take advantage of the recently developed instrumentation and the vast array of novel probes that have led to advances in the field of flow cytometry, as well as to significantly improved standard methods. This eminently practical new book will play a key role in bringing researchers up-to-date in this unique and productive technology.
988			_aSpringer_Protocols_1998
650		7	_2embne _9668358 _aCitometría de flujo
776	0	8	_iPrinted edition: _z9781489942692
776	0	8	_iPrinted edition: _z9780896033542
776	0	8	_iPrinted edition: _z9781489942685
776	0	8	_iPrinted edition: _z9780896035386
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896033546 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _dz _ean _zSI