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008			100301s2001 xxu| fo |||| 0|eng d
020			_a9781592591183
024	7		_a10.1385/1592591183 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH603 .I54 _b2001 EB
245	0	0	_aIon Channel Localization _cedited by Anatoli Lopatin, Colin G. Nichols
250			_a1st edition 2001
264		1	_aTotowa, NJ _bHumana Press _c2001
300			_a1 recurso en línea (XVII, 502 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Pharmacology and Toxicology _x1940-6053
505	0		_aPharmacological Labeling of Ion Channels and Receptors -- Fluorescent Calcium Antagonists Tools for Imaging of L-Type Calcium Channels in Living Cells -- Targeting Cerebral Muscarinic Acetylcholine Receptors with Radioligands for Diagnostic Nuclear Medicine Studies -- GABAA Receptors -- Localization of ATP P2X Receptors -- Small Conductance Calcium-Activated Potassium Channels in Rat Brain -- Mapping N-Type Calcium Channel Distributions with H-Conotoxins -- Fluorescent Imaging of Nicotinic Receptors During Neuromuscular Junction Development -- High Affinity Scorpion Toxins for Studying Potassium and Sodium Channels -- Applications of Green Fluorescent Protein (GFP) Technology -- Bicistronic GFP Expression Vectors as a Tool to Study Ion Channels in Transiently Transfected Cultured Cells -- Confocal Imaging of GFP-Labeled Ion Channels -- Localization and Quantification of GFP-Tagged Ion Channels Expressed in Xenopus Oocytes -- Applications of Green Fluorescent Protein (GFP) Technology -- Tagging Ion Channels with the Green Fluorescent Protein (GFP) as a Method for Studying Ion Channel Function in Transgenic Mouse Models -- Mutant GFP- Based FRET Analysis of K+ Channel Organization -- Delivering Ion Channels to Mammalian Cells by Membrane Fusion -- Functional Assays in Ion Channel Localization -- Detection of Neurons Expressing Calcium- Permeable AMPA Receptors Using Kainate-Induced Cobalt Uptake -- Crystallization Technique for Localizing Ion Channels in Living Cells -- Imaging of Localized Neuronal Calcium Influx -- Using Caged Compounds to Map Functional Neurotransmitter Receptors -- Atomic Force Microscopy -- Atomic Force Microscopy of Reconstituted Ion Channels -- Imaging the Spatial Organization of Calcium Channels in Nerve Terminals Using Atomic Force Microscopy -- Nanoarchitecture of Plasma Membrane Visualized with Atomic Force Microscopy -- Dynamic AFM of Patch Clamped Membranes -- Localizing Ion Channels with Scanning Probe Microscopes: A Perspective.
520			_aAlthough there are many useful books on virtually every aspect of ion channel structure and function, work on channel localization-determining the specific sites of ion channels and their receptors on cells-has long been neglected. In Ion Channel Localization: Methods and Protocols, active researchers at the cutting-edge of their fields describe how to use current and developing technologies to determine the exact cell sites of such channels and receptors. With emphasis on techniques employing the unique properties of these proteins, the core topics of the book include all the major pharmacological tools for ion channel determination. Also covered are fluorescent and radioligand assays, exciting new visualization methods employing green fluorescent protein (GFP), and novel assays based on the functional properties of ion channels and receptors, as well as rapidly developing atomic force microscopy methodologies. Comprehensive and rich in practical detail, Ion Channel Localization: Methods and Protocols brings together in a single volume detailed reviews of all the major techniques-both currently in use and in development-for localizing ion channels and receptors in cells, tissues, and organisms.
988			_aSpringer_Protocols_2001
650		7	_2embne _9155847 _aCanales iónicos
776	0	8	_iPrinted edition: _z9781617372117
776	0	8	_iPrinted edition: _z9780896038332
776	0	8	_iPrinted edition: _z9781489941978
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592591183 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _dz _ean _zSI