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008			130718s2013 xxu| fo |||| 0|eng d
020			_a9781627035385
024	7		_a10.1007/978-1-62703-538-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP551 _b2013 EB
245	0	0	_aAdhesion Protein Protocols _cedited by Amanda S. Coutts
250			_a3rd edition 2013
264		1	_aTotowa, NJ _bHumana Press _c2013
300			_a1 recurso en línea (XII, 407 páginas) _b87 ilustraciones, 57 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1046
505	0		_aReconstructing Integrin Activation in vitro -- Quantifying Cellular Adhesion to Covalently Immobilized Extracellular Matrix Proteins by Single-Force Spectroscopy -- A Multimode-TIRFM & Microfluidic Technique to Examine Platelet Adhesion Dynamics -- Localization-Based Super-Resolution Imaging of Cellular Structures -- Use of Microarray Analysis to Investigate EMT Gene Signatures.-  Podosome Reformation in Macrophages: Assays and Analysis -- Mobile and Three-Dimensional Presentation of Adhesion Proteins Within Microwells -- Basement Membrane Invasion Assays: Native Basement Membrane and Chemoinvasion Assay -- Cytoplasmic Actin: Purification and Single Molecule Assembly Assays -- Synthetic and Tissue-Derived Models for Studying Rigidity Effects on Invadopodia Activity -- Determining Rho GTPase Activity by an Affinity-Precipitation Assay -- Proteomic and Biochemical Methods to Study the Cytoskeletome. Fabricating Surfaces with Distinct Geometries and Different Combinations of Cell Adhesion Proteins -- Purification of Native Arp2/3 Complex from Bovine Thymus -- Purification of Arp2/3 Complex from Saccharomyces Cerevisiae -- Measurement and Analysis of in vitro Actin Polymerization -- Use of the xCELLigence System for Real Time Analysis of changes in Cellular Motility and Adhesion in Physiological Conditions -- Measuring Chemotaxis Using Direct Visualisation Microscope Chambers -- In Vitro Microtubule Severing Assays -- VE-Cadherin Status as an Indicator of Microvascular Permeability -- High-Resolution Live-Cell Imaging and Time-Lapse Microscopy of Invadopodium Dynamics and Tracking Analysis -- Live Cell Imaging of RhoGTPase Biosensors in Tumor Cells -- Electrospun Nanofiber Scaffolds for Investigating Cell-matrix Adhesion -- Method for Measuring Single-Molecule Adhesion Forces and Attachment Lifetimes of Protein-Membrane Interactions -- VE-Cadherin Status as an Indicator of Microvascular Permeability -- High-Resolution Live-Cell Imaging and Time-Lapse Microscopy of Invadopodium Dynamics and Tracking Analysis. Live Cell Imaging of RhoGTPase Biosensors in Tumor Cells -- Electrospun Nanofiber Scaffolds for Investigating Cell-matrix Adhesion -- Method for Measuring Single-Molecule Adhesion Forces and Attachment Lifetimes of Protein-Membrane Interactions.
520			_aCellular adhesion is a fundamental process that influences numerous biological activities such as morphogenesis, cell motility and division, as well as signalling. In addition, adhesion is a process important not only in normal physiology and development, but also in disease states such as tumourigenesis, cardiovascular disease, inflammation and infection. There are a plethora of proteins involved in adhesion-related events with a huge diversity in function. As a result, a wide variety of techniques exist to study adhesion related proteins and processes. In Adhesion Protein Protocols, Third Edition, chapters cover techniques to gain insight into the complex and incompletely understood processes that are involved in cellular adhesion. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, Adhesion Protein Protocols, Third Edition will be useful for both those new to the field of adhesion protein research as well as the more experienced scientist.
988			_aSpringer_Protocols_2013
650		7	_2embne _9242917 _aProteínas _xAnálisis
776	0	8	_iPrinted edition: _z9781627035392
776	0	8	_iPrinted edition: _z9781627035378
776	0	8	_iPrinted edition: _z9781493963119
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-538-5 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b10/2023 _dz _ean _zSI