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008			110520s2011 xxu| o |||| 0|eng d
020			_a9781617791116
024	7		_a10.1007/978-1-61779-111-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP551 _b2011 EB
245	0	0	_aNeuroproteomics _cedited by Ka Wan Li
250			_a1st edition 2011
264		1	_aTotowa, NJ _bHumana Press _c2011
300			_a1 recurso en línea (XIII, 317 páginas) _b76 ilustraciones, 17 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aNeuromethods _x1940-6045 _v57
505	0		_aNeuroproteomics: Deciphering Brain Function and Disorders -- Dissection of Rodent Brain Regions -- Subcellular Fractionation of Brain Tissue Using Free Flow Electrophoresis -- Isolation of Synapse Sub-Domains by Subcellular Fractionation Using Sucrose Density Gradient Centrifugation -- Enrichment of Plasma Membranes from Small Brain Samples by Aqueous Polymer Two-Phase Systems -- Identification and Characterization of Protein Complexes from Total Brain and Synaptoneurosomes: Heterogeneity of Molecular Complexes in Distinct Subcellular Domains -- Analysis of Protein Complexes by 2D Blue Native/SDS-PAGE and Antibody-Shift Assay -- 2-Dimensional Gel Electrophoresis-Based Proteomic Analysis of Brain Synapses -- 2-Dimensional BAC-SDS Polyacrylamide Gel Electrophoresis for the Fractionation and Identification of Synaptic Vesicle Proteins and the Presynaptic Active Zone -- iTRAQ-Based LC-LC MALDI TOF/TOF MS Quantitative Analysis of Membrane Proteins from Human Glioma -- OFFGEL-Isoelectric Focusing Fractionation for the Analysis of Complex Proteome -- A 1D-PAGE/LC-ESI Linear Ion Trap Orbitrap MS Approach for the Analysis of Synapse Proteomes and Synaptic Protein Complexes -- SDS-PAGE Immuno-Blot Analysis -- Phosphoproteomics by Highly Selective IMAC Protocol -- Global Analysis of Ubiquitination -- High-Throughput High-Content Functional Image Analysis of Neuronal Proteins Implicated in Parkinson's Disease -- Neuropeptidomics of the Mammalian Brain -- In-Depth Analysis of the Cerebrospinal Fluid Proteome and Biomarker Discovery: Abundant Protein Depletion Sample Pretreatment Method -- Lipidomics of the Nervous System: Phospholipidomics by Liquid Chromatography Coupled to Mass Spectrometry or Tandem Mass Spectrometry -- Bioinformatics Procedures for Analysis of Quantitative Proteomics Experiments Using iTRAQ -- Statistical Analysis of Spectral Count Data Generated by Label-Free Tandem Mass Spectrometry-Based Proteomics.
520			_aIn the past few years, the application of proteomics to examine the molecular mechanisms underlying (mal-)functioning of the nervous system and brain disorders has risen steeply, which in many cases has yielded novel insights. As neuroproteomics is a new research field involving the use of a number of high-end analytical instruments and technologies, both promises and pitfalls may not be well appreciated by the researchers, and the design of a proper proteomics experiment may not be obvious.  Neuroproteomics brings together a collection of detailed protocols written by experienced professionals in the field in order to cover aspects of various dimensions of this new technology, together with some established methods that have supporting roles in the workflow of neuroproteomics. Written for the popular Neuromethods series, the chapters in this book contain step-by-step, easy to follow instruction along with practical tips and key implementation advice essential for successful implementation of the techniques.   Authoritative and cutting-edge, Neuroproteomics will be of great assistance to all researchers seeking to design and execute their own proteomics experiments in an optimal way and continue moving forward this exciting area of study.
988			_aSpringer_Protocols_2011
650		7	_2embne _9430647 _aProteómica
650		7	_2embne _9138462 _aSistema nervioso
776	0	8	_iPrinted edition: _z9781617791109
776	0	8	_iPrinted edition: _z9781617791123
776	0	8	_iPrinted edition: _z9781493958344
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-111-6 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b01/2024 _dz _eIG _zSI