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008			140607s2014 xxu| o |||| 0|eng d
020			_a9781493909285
024	7		_a10.1007/978-1-4939-0928-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR185.8 .C95 _b2014 EB
245	0	0	_aCytokine Bioassays : _bMethods and Protocols _cedited by Ivana Vancurova
250			_a1st edition 2014
264		1	_aNew York, NY _bSpringer International Publishing _c2014
300			_a1 recurso en línea (XIV, 367 páginas) _b87 ilustraciones, 74 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1172
505	0		_aThe Challenge of Measuring Elusive Immune Markers by Enzyme Linked Immuno-Spot (ELISPOT) Technique -- Analysis of the Released Nuclear Cytokine HMGB1 in Human Serum -- αELISA Based Assay for IP-10 Detection from Filter Paper Samples -- Enhanced ELISA Based on Carboxymethylated Dextran Coatings -- A Novel Three-Dimensional Biosensor Based on Aluminum Oxide: Application for Early Stage Detection of Human Interleukin-10 -- Quantification of Multiple Cytokines and Chemokines Using Cytometric Bead Arrays -- A Useful Guide for Analysis of Immune Markers by Fluorochrome (Luminex) Technique -- Control of Pro-inflammatory Cytokine Release from Human Monocytes with the Use of an Interleukin-10 Monoclonal Antibody -- Cytokine-Induced Neutrophil Chemotaxis Assay -- An In Vitro One-Dimensional Assay to Study Growth Factor-Regulated Tumor Cell-Macrophage Interaction -- Chemotactic Responses by Macrophages to a Directional Source of a Cytokine Delivered by a Micropipette -- Assessment of Phagocytic Activity of Cultured Macrophages Using Fluorescence Microscopy and Flow Cytometry -- Assessment of Cytokine-Modulated Proteasome Activity -- Evaluation of the Adverse Effect of Low Concentration of Cadmium on Interleukin-4 Induced Class Switch Recombination in Burkett's Lymphoma Raji Cell Line -- A Mix-and-Measure Assay for Determining the Activation Status of Endogenous Cdc42 in Cytokine-Stimulated Macrophage Cell Lysates -- Analysis of the Cell Surface Expression of Cytokine Receptors Using the Surface Protein Biotinylation Method -- Detection of CXCR2 Cytokine Receptor Surface Expression Using Immunofluorescence -- Detecting Tie2, an Endothelial Growth Factor Receptor, by Using Immunohistochemistry in Mouse Lungs -- Use of shRNA to Stably Suppress Chemokine Receptor Expression and Function in Human Cancer Cell Lines -- Intracellular Staining and Detection of Cytokines by Fluorescence-Activated Flow Cytometry -- Cytokine Detection by Flow Cytometry -- Analysis of IL-17 Production by Flow Cytometry and ELISPOT Assays -- Interleukin-1 (IL-1) Immunohistochemistry Assay in Oral Squamous Cell Carcinoma -- Immunofluorescence Confocal Microscopy of Intracellular TNF in Human Neutrophils -- Evaluating Cytoplasmic and Nuclear Levels of Inflammatory Cytokines in Cancer Cells by Western Blotting -- Western Analysis of Intracellular Interleukin-8 in Human Mononuclear Leukocytes -- Quantitative Analysis of Bortezomib Induced IL-8 Gene Expression in Ovarian Cancer Cells -- Analysis of TGFb1 and IL-10 Transcriptional Regulation in CTCL Cells by Chromatin Immunoprecipitation -- Radiolabeled Semi-Quantitative RT-PCR Assay for the Analysis of Alternative Splicing of Interleukin Genes.
520			_aCytokine Bioassays: Methods and Protocols provides a comprehensive collection of classic and cutting-edge methodologies that are used to analyze and quantify cytokines and their biological activities in complex biological and clinical samples. Chapters are divided into three main categories, the first category details the immunodetection of released cytokines in tissue culture supernatants, plasma, serum, and whole blood samples by immunoassays. The second part focuses on the analysis of biologically active cytokines by bioassays using neutralizing antibodies, chemotaxis assay, cytokine-induced phagocytosis assay, proteasome activity assay, and analysis of cytokine-induced immunoglobulin class switching. Part three presents analysis of intracellular cytokines by flow cytometry, immunohistochemistry, immunofluorescence confocal microscopy, and western blotting. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cytokine Bioassays: Methods and Protocols is written by experienced, basic, and clinical researchers with hands-on knowledge of the described protocols. This book will be of interest not only to biochemists, molecular biologists and immunologists but also to physician-scientists working in the field of cytokine research.
988			_aSpringer_Protocols_2014
650		7	_2embne _9147650 _aCitoquinas
776	0	8	_iPrinted edition: _z9781493909292
776	0	8	_iPrinted edition: _z9781493909278
776	0	8	_iPrinted edition: _z9781493948505
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-0928-5 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
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998			_b01/2024 _dz _eIG _zSI