000			05583nam a22004095i 4500
999			_c392744 _d392744 _x1
001			392744
003			ES-MaUEC
005			20240314174305.0
006			a|||| o|||| 00| 0
007			cr nn 008mamaa
008			100301s1998 xxu| o |||| 0|eng d
020			_a9781592595662
024	7		_a10.1385/0896033856 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR351 _b1998 EB
245	0	0	_aPlant Virology Protocols : _bFrom Virus Isolation to Transgenic Resistance _cedited by Gary D. Foster, Sally Taylor
250			_a1st edition 1998
264		1	_aTotowa, NJ _bHumana Press _c1998
300			_a1 recurso en línea (XV, 571 páginas) _b111 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v81
505	0		_ato Plant Virology -- to Classical Crossprotection -- History of Coat Protein-Mediated Protection -- Virus Isolation, RNA Extraction, and Location of Coat Protein Genes -- Geminivirus Isolation and DNA Extraction -- Caulimovirus Isolation and DNA Extraction -- Reovirus Isolation and RNA Extraction -- Procedures for Plant Rhabdovirus Purification, Polyribosome Isolation, and Replicase Extraction -- Hordeivirus Isolation and RNA Extraction -- Furovirus Isolation and RNA Extraction -- Tobravirus Isolation and RNA Extraction -- Tobamovirus Isolation and RNA Extraction -- Potexvirus Isolation and RNA Extraction -- Carlavirus Isolation and RNA Extraction -- Potyvirus Isolation and RNA Extraction -- Trichovirus Isolation and RNA Extraction -- Ilarvirus Isolation and RNA Extraction -- Bromovirus Isolation and RNA Extraction -- Cucumovirus Isolation and RNA Extraction -- Nepovirus Isolation and RNA Extraction -- Comovirus Isolation and RNA Extraction -- Carmovirus Isolation and RNA Extraction -- Tymovirus Isolation and Genomic RNA Extraction -- Tombusvirus Isolation and RNA Extraction -- Luteovirus Isolation and RNA Extraction -- Cloning of Coat Protein Genes -- RNA Analysis -- RNA Fractionation by Density Gradient Centrifugation -- cDNA Library Construction for the Lambda ZAP®-Based Vectors -- PCR Cloning of Coat Protein Genes -- Antibody Production -- Expression Library Screening -- In Vitro Transcription and Translation -- Analysis of Coat Protein Expression Cassettes in Protoplasts -- DNA Sequencing -- Computer Analysis of Amino Acid Sequences -- Plant Transformation and Primary Analysis -- Preparation of Coat Protein-Containing Binary Vectors for Use in Agrobacterium-Mediated Transformation -- Potato Transformation -- Transformation of Tomato -- Tobacco Transformation -- Genetic Transformation of Wheat -- Production of Transgenic Rice (Oryza sativa subspecies japonica cv. Taipei 309) -- Molecular Analysis of Transgenic Rice -- PCR Analysis of Transgenic Tobacco Plants -- Southern Analysis of Transgenic Tobacco Plants -- Detection and Quantification of Transcript RNA in Transgenic Plants Using Digoxigenin-Labeled cDNA Probes -- Western Analysis of Transgenic Plants -- Evaluation of Resistance -- Assaying Levels of Plant Virus by ELISA -- Detection of Plant RNA Viruses by Nonisotopic Dot-Blot Hybridization -- Detection and Quantification of Plant Viruses by PCR -- Assaying Levels of Virus with Local Lesion Hosts -- Field Testing Resistance of Transgenic Plants -- Agronomic Performance of Transgenic Plants -- Mechanisms of Resistance -- Mechanisms of Resistance -- Mechanisms of RNA-Mediated Resistance to Plant Viruses -- Detection of Risks Associated with Coat Protein Transgenics -- Potential Benefits of the Transgenic Control of Plant Viruses in the United Kingdom.
520			_aPlant Virology Protocols offers for the first time a comprehensive collection of state-of-the-art techniques for generating transgenic plants that are resistant to plant viruses via the cloning and expression of the coat protein gene. Its unfailingly reproducible methods, perfected by hands-on masters, cover the entire process from virus isolation, RNA extraction, and cloning coat protein genes, to the introduction of the coat protein gene into the plant genome and the testing of transgenic plants for resistance. Methods for testing for transformation by PCR and Southern blotting, the detection of RNA transcripts by Northern blotting, and the production of protein by Western analysis are provided, as are methods for challenging the transgenic plants produced and for detecting and measuring the levels of virus. The authoritative contributors also discuss the history and mechanisms of coat protein-mediated protection and the key ethical issues surrounding transgenic technology. Unprecedented in its comprehensiveness and its many time-proven methods, Plant Virology Protocols is certain to become the authoritative standard reference for plant scientists who want to tap the enormous benefits this technology has to offer.
988			_aSpringer_Protocols_1998
650		7	_2embne _9676135 _aVirología molecular
650		7	_2embne _9145477 _aEnfermedades víricas en plantas
776	0	8	_iPrinted edition: _z9781489942531
776	0	8	_iPrinted edition: _z9780896033856
776	0	8	_iPrinted edition: _z9781489942524
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896033856 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b01/2024 _dz _eIG _zSI