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020 _a9781627039895
024 7 _a10.1007/978-1-62703-989-5
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQL821
_b2014 EB
245 0 0 _aSkeletal Development and Repair :
_bMethods and Protocols
_cedited by Matthew J. Hilton
250 _a1st edition 2014
264 1 _aTotowa, NJ
_bHumana Press
_c2014
300 _a1 recurso en línea (X, 328 páginas)
_b78 ilustraciones, 40 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1130
505 0 _aOverview of Skeletal Development -- Overview of Skeletal Repair (Fracture Healing and Its Assessment) -- Generation of Closed Transverse Fractures in Small Animals -- The Murine Femoral Bone Graft Model and a Semi-Automated Histomorphometric Analysis Tool -- Surgical Induction of Posttraumatic Osteoarthirits -- A Mouse Model of Flexor Tendon Repair -- Cartilage Explant Cultures -- Renal Capsule Transplantations to Assay Skeletal Angiogenesis -- Whole Mount Skeletal Staining -- Tips and Techniques for Processing and Sectioning Undecalcified Murine Bone Specimens -- Preparation of Thin Sections from Non-fixed and Undecalcified Hard Tissues Using Kawamoto's Film Method (2012) -- Demineralized Murine Skeletal Histology -- Beta-Galactosidase Staining in the Skeleton -- Whole-Mount In Situ Hybridization on Murine Skeletogenic Tissues -- Non-Radioactive In Situ Hybridization on Skeletal Tissue Sections -- Radioactive In Situ Hybridization to Detect Gene Expression in Skeletal Tissue Sections -- Proliferation Assays (BrdU and EdU) on Skeletal Tissue Sections -- TUNEL Assay on Skeletal Tissue Sections to Detect Apoptosis -- Analysis of Chondrogenesis Using Micromass Cultures of Limb Mesenchyme -- Isolation and Culture of Murine Primary Chondrocytes -- Bone Marrow Stromal Cell Assays - In Vitro and In Vivo -- Isolation and Culture of Neonatal Mouse Calvarial Osteoblasts -- RANKL-Based Osteoclastogenic Assays from Murine Bone Marrow Cells -- Hematopoietic Stem Cell Cultures and Assays.
520 _aSkeletal Development and Repair: Methods and Protocols is a compilation of a variety of skeletal research protocols utilizing the laboratory mouse as the platform for surgical manipulation and/or transplantation, as well as, the source of tissues and cells for in vitro culture and analyses. Chapters are written by experts in the field and cover topics including surgical, transplantation, and organ culture methods that permit analyses of skeletal tissues undergoing repair in vivo and permits analyses of cellular interactions ex vivo, histological and molecular techniques developed to study gene and protein expression in whole embryos, skeletal tissues and tissue sections, and in vitro primary cell culture protocols designed to assay gene function in specific cell populations. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, Skeletal Development and Repair: Methods and Protocols is a comprehensive laboratory manual for all levels of basic research scientists working in the broad fields of skeletal development and skeletal repair research.
988 _aSpringer_Protocols_2014
650 7 _2embne
_9666561
_aHuesos
650 7 _2embne
_9138515
_aAnimales de laboratorio
650 7 _2embne
_9139725
_aEsqueleto
776 0 8 _iPrinted edition:
_z9781627039901
776 0 8 _iPrinted edition:
_z9781627039888
776 0 8 _iPrinted edition:
_z9781493963102
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-989-5
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b01/2024
_dz
_eIG
_zSI