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008			100301s2002 xxu| o |||| 0|eng d
020			_a9781592591855
024	7		_a10.1385/159259185X _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP88.4 _b2002 EB
245	0	0	_aEpithelial Cell Culture Protocols _cedited by Clare Wise
250			_a1st edition 2002
264		1	_aTotowa, NJ _bHumana Press _c2002
300			_a1 recurso en línea (XIV, 406 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v188
505	0		_aHuman Lens Epithelial Cell Culture -- Human Airway Epithelial Cell Culture -- Rat Gastric Mucosal Epithelial Cell Culture -- Thymic Epithelial Cell Culture -- Bile Duct Epithelial Cell Culture -- Liver Epithelial Cell Culture -- Pulmonary Epithelial Cell Culture -- Prostate Epithelial Cell Isolation and Culture -- A Bovine Mammary Endothelial/Epithelial Cell Culture Model of the Blood/Milk Barrier -- The Blood-CSF Barrier in Culture -- An In Vitro Model of Differentiated Human Airway Epithelia -- Primary Mouse Keratinocyte Culture -- Analyzing Apoptosis in Cultured Epithelial Cells -- Keratins as Markers of Epithelial Cells -- Epithelial Cell Integrins -- Isolation, Cultivation, and Differentiation of Normal Human Epidermal Keratinocytes in Serum-Free Medium -- Clinical Application of Autologous Cultured Epithelia for the Treatment of Burns and Disfigurement of Skin Surfaces -- Transplantation of Cultured Epithelial Cells -- An Outgrowth Culture System of Normal Human Epithelium -- Application of Epithelial Cell Culture in Drug Transport in the Respiratory Tract -- Applications of Epithelial Cell Culture in Studies of Drug Transport -- X-Ray Microanalysis of Epithelial Cells in Culture -- ENU Mutagenesis of Rat Mammary Epithelial Cells -- ENU-Induced Ovarian Cancer -- Measurement of Membrane Capacitance in Epithelial Monolayers -- Assessing Epithelial Cell Confluence by Spectroscopy -- Co-Cultivation of Liver Epithelial Cells with Hepatocytes -- Co-Culture and Crosstalk between Endothelial Cells and Vascular Smooth Muscle Cells Mediated by Intracellular Calcium -- Establishing Epithelial-Immune Cell Co-Cultures -- Explant Cultures of Embryonic Epithelium -- Bacterial Interactions with Host Epithelium In Vitro.
520			_aMany powerful new techniques for the isolation and culture of epithelial cells have been developed in the past decade. In Epithelial Cell Culture Protocols, a team of well-versed experimenters and clinical researchers share their best methods for establishing and maintaining epithelial cell cultures, for analyzing and studying their characteristics, and for using them to set up models of critical biological systems. These readily reproducible techniques describe all the commonly used protocols, as well as those that are more specialized, including several aimed at clinical investigation. The emphasis is on the analysis and assessment of epithelial cells, for example, by looking at apoptosis and integrins or by measuring membrane capacitance and confluence. Also described in step-by-step detail are co-culture techniques valuable in developing models for investigating many different in vitro systems, including the blood-brain barrier, drug uptake, and the interaction of epithelial cells with bacteria. Several protocols cover the culturing of epithelial cells and their use in treating patients with burns and other skin disorders. Wide-ranging and highly practical, Epithelial Cell Culture Protocols offers both novices and expert investigators alike a step-by-step guide toward a deeper understanding of cellular and molecular mechanisms in general, as well as a set of robust techniques for specifically evaluating the nature and behavior of epithelial cells.
988			_aSpringer_Protocols_2002
650		7	_2embne _9668849 _aTejidos (Biología)
650		7	_2embne _9151163 _aEpitelio
776	0	8	_iPrinted edition: _z9781617372414
776	0	8	_iPrinted edition: _z9781489938824
776	0	8	_iPrinted edition: _z9780896038936
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/159259185X _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b01/2024 _dz _eIG _zSI