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008			100301s2007 xxu| o |||| 0|eng d
020			_a9781597454575
024	7		_a10.1007/978-1-59745-457-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR398.5 _b2007 EB
245	0	0	_aBaculovirus and Insect Cell Expression Protocols _cedited by David W. Murhammer
250			_a2nd edition 2007
264		1	_aTotowa, NJ _bHumana Press _c2007
300			_a1 recurso en línea (XIV, 470 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v388
505	0		_aUseful Tips, Widely Used Techniques, and Quantifying Cell Metabolic Behavior -- Baculovirus Molecular Biology/Development of Recombinant Baculoviruses -- to Baculovirus Molecular Biology -- Baculovirus Transfer Vectors -- Recombinant Baculovirus Isolation -- Gene Expression in Mammalian Cells Using BacMam, a Modified Baculovirus System -- Insect Cell Culture -- Available Lepidopteran Insect Cell Lines -- Lepidopteran Insect Cell Line Isolation from Insect Tissue -- Development of Serum-Free Media for Lepidopteran Insect Cell Lines -- Routine Maintenance and Storage of Lepidopteran Insect Cell Lines and Baculoviruses -- Protein Production with Recombinant Baculoviruses -- Small-Scale Protein Production With the Baculovirus Expression Vector System -- Recombinant Protein Production in Large-Scale Agitated Bioreactors Using the Baculovirus Expression Vector System -- Production of Baculovirus-Expressed Recombinant Proteins in Wave Bioreactors -- Protein Production With Recombinant Baculoviruses in Lepidopteran Larvae -- Virus-like Particle and Viral Vector Production Using the Baculovirus Expression Vector System/Insect Cell System -- Recombinant Protein Production with Transformed Insect Cells -- Transforming Lepidopteran Insect Cells for Continuous Recombinant Protein Expression -- Transformation of Drosophila Cell Lines -- Transforming Lepidopteran Insect Cells for Improved Protein Processing -- Baculovirus Development and Production for Use as Insecticides -- to the Use of Baculoviruses as Biological Insecticides -- Baculovirus Insecticide Production in Insect Larvae -- Evaluation of the Insecticidal Efficacy of Wild-Type and Recombinant Baculoviruses -- Miscellaneous Techniques and Applications of the Baculovirus/Insect Cell System -- Monitoring and Visualization of Baculovirus Infection Using Green Fluorescent Protein Strategy -- Alternative Bioreactor Strategy for Probing Infection and Production -- Methods for Gene Silencing With RNAi -- Using the Baculovirus/Insect Cell System to Study Apoptosis -- Generation of Envelope-Modified Baculoviruses for Gene Delivery Into Mammalian Cells.
520			_aBaculovirus Expression Protocols is a detailed guide for using the baculovirus expression vector system (BEVS) and/or insect cells to produce recombinant proteins. Procedures for the development and production of baculovirus insecticides at both laboratory and large scale are described in the kind of step-by-step format that the Methods in Molecular Biology™ series helped standardize. This book is a one-stop source for information on baculoviruses, and includes sections on using modified baculoviruses to express genes in mammalian cells, using Drosophila cell lines, isolating new cell lines, and developing serum-free medium. Specific applications, including using baculovirus and insect cell system to study apoptosis, are also discussed. This updated and expanded edition of Baculovirus Expression Protocols provides a perfect lab manual for biochemists, molecular biologists, biochemical engineers, and others for using the BEVS or insect cell lines to produce recombinant proteins.
988			_aSpringer_Protocols_2007
650		7	_2embne _9689398 _aBaculovirus
650		7	_2embne _9141520 _aIngeniería genética
776	0	8	_iPrinted edition: _z9781617376290
776	0	8	_iPrinted edition: _z9781588295378
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-457-5 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b01/2024 _dz _eIG _zSI