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008			100715s2010 xxu| o |||| 0|eng d
020			_a9781607617839
024	7		_a10.1007/978-1-60761-783-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aRL106 .E44 _b2010 EB
245	0	0	_aImmunoelectron Microscopy : _bMethods and Protocols _cedited by Steven D. Schwartzbach, Tetsuaki Osafune
250			_a1st edition 2010
264		1	_aTotowa, NJ _bHumana Press _c2010
300			_a1 recurso en línea (XI, 352 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v657
505	0		_aMolecular Toolbox -- Protein Antigen Expression in Escherichia coli for Antibody Production -- Expression of Epitope-Tagged Proteins in Plants -- Expression of Epitope-Tagged Proteins in Arabidopsis Leaf Mesophyll Protoplasts -- Transient Expression of Epitope-Tagged Proteins in Mammalian Cells -- Production and Purification of Polyclonal Antibodies -- Production and Purification of Monoclonal Antibodies -- Production of Antipeptide Antibodies -- Preparation of Colloidal Gold Particles and Conjugation to Protein A, IgG, F(ab')2, and Streptavidin -- Microscopy Toolbox -- Immunoelectron Microscopy of Chemically Fixed Developing Plant Embryos -- Pre-embedding Immunogold Localization of Antigens in Mammalian Brain Slices -- Pre-embedding Immunoelectron Microscopy of Chemically Fixed Mammalian Tissue Culture Cells -- Immunoelectron Microscopy of Cryofixed and Freeze-Substituted Plant Tissues -- In Vivo Cryotechniques for Preparation of Animal Tissues for Immunoelectron Microscopy -- Immunoelectron Microscopy of Cryofixed Freeze-Substituted Mammalian Tissue Culture Cells -- Immunoelectron Microscopy of Cryofixed Freeze-Substituted Saccharomyces cerevisiae -- High-Resolution Molecular Localization by Freeze-Fracture Replica Labeling -- Pre-embedding Electron Microscopy Methods for Glycan Localization in Chemically Fixed Mammalian Tissue Using Horseradish Peroxidase-Conjugated Lectin -- Pre-embedding Nanogold Silver and Gold Intensification -- The Post-embedding Method for Immunoelectron Microscopy of Mammalian Tissues: A Standardized Procedure Based on Heat-Induced Antigen Retrieval -- Double-Label Immunoelectron Microscopy for Studying the Colocalization of Proteins in Cultured Cells -- Serial Section Immunoelectron Microscopy of Algal Cells -- Freeze-Etch Electron Tomography for the Plasma Membrane Interface -- Localization of rDNA at Nucleolar Structural Components by Immunoelectron Microscopy -- Immunogold Labelling for Scanning Electron Microscopy -- Horseradish Peroxidase as a Reporter Gene and as a Cell-Organelle-Specific Marker in Correlative Light-Electron Microscopy -- Monitoring Rapid Endocytosis in the Electron Microscope via Photoconversion of Vesicles Fluorescently Labeled with FM1-43.
520			_aImmunoelectron microscopy is a key technique that bridges the information gap between biochemistry, molecular biology, and ultrastructural studies placing macromolecular functions within a cellular context. In Immunoelectron Microscopy: Methods and Protocols, expert researchers combine the tools of the molecular biologist with those of the microscopist. From the molecular biology toolbox, this volume presents methods for antigen production by protein expression in bacterial cells, methods for epitope tagged protein expression in plant and animal cells allowing protein localization in the absence of protein specific antibodies as well as methods for the production of anti-peptide, monoclonal, and polyclonal antibodies. From the microscopy toolbox, sample preparation methods for cells, plant, and animal tissue are presented. Both cryo-methods, which have the advantage of retaining protein antigenicity at the expense of ultrastructural integrity, as well as chemical fixation methods that maintain structural integrity while sacrificing protein antigenicity have been included, with chapters examining various aspects of immunogold labeling. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and essential, Immunoelectron Microscopy: Methods and Protocols seeks to facilitate an increased understanding of structure function relationships.
988			_aSpringer_Protocols_2010
650		7	_2embne _9138721 _aMicroscopia electrónica
776	0	8	_iPrinted edition: _z9781607617822
776	0	8	_iPrinted edition: _z9781607617846
776	0	8	_iPrinted edition: _z9781493957583
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60761-783-9 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
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998			_b01/2024 _dz _eIG _zSI