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007			cr nn 008mamaa
008			100301s2002 xxu| o |||| 0|eng d
020			_a9781592591770
024	7		_a10.1385/1592591779 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH442.2 _b2002 EB
245	0	0	_aPCR Cloning Protocols _cedited by Bing-Yuan Chen, Harry W. Janes
250			_a2nd edition 2002
264		1	_aTotowa, NJ _bHumana Press _c2002
300			_a1 recurso en línea (XIV, 439 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v192
505	0		_aPerforming and Optimizing PCR -- Polymerase Chain Reaction -- Computer Programs for PCR Primer Design and Analysis -- Single-Step PCR Optimization Using Touchdown and Stepdown PCR Programming -- XL PCR Amplification of Long Targets from Genomic DNA -- Coupled One-Step Reverse Transcription and Polymerase Chain Reaction Procedure for Cloning Large cDNA Fragments -- Long Distance Reverse-Transcription PCR -- Increasing PCR Sensitivity for Amplification from Paraffin-Embedded Tissues -- GC-Rich Template Amplification by Inverse PCR -- PCR Procedure for the Isolation of Trinucleotide Repeats -- Methylation-Specific PCR -- Direct Cloning of Full-Length Cell Differentially Expressed Genes by Multiple Rounds of Subtractive Hybridization Based on Long-Distance PCR and Magnetic Beads -- Cloning PCR Products -- Cloning PCR Products -- Using T4 DNA Polymerase to Generate Clonable PCR Products -- Enzyme-Free Cloning of PCR Products and Fusion Protein Expression -- Directional Restriction Site-Free Insertion of PCR Products into Vectors -- Autosticky PCR -- A Rapid and Simple Procedure for Direct Cloning of PCR Products into Baculoviruses -- Mutagenesis and Recombination -- PCR Approaches to DNA Mutagenesis and Recombination -- In-Frame Cloning of Synthetic Genes Using PCR Inserts -- Megaprimer PCR -- PCR-Mediated Recombination -- PCR Method for Generating Multiple Mutations at Adjacent Sites -- A Fast Polymerase Chain Reaction-Mediated Strategy for Introducing Repeat Expansions into CAG-Repeat Containing Genes -- PCR Screening in Signature-Tagged Mutagenesis of Essential Genes -- Staggered Extension Process (StEP) In Vitro Recombination -- Random Mutagenesis by Whole-Plasmid PCR Amplification -- Cloning Unknown Neighboring DNA -- PCR-Based Strategies to Clone Unknown DNA Regions from Known Foreign Integrants -- Long Distance Vectorette PCR (LDV PCR) -- Nonspecific, Nested Suppression PCR Method for Isolation of Unknown Flanking DNA ("Cold-Start Method") -- Inverse PCR -- 31 Inverse PCR -- Gene Cloning and Expression Profiling by Rapid Amplification of Gene Inserts with Universal Vector Primers -- The Isolation of DNA Sequences Flanking Tn5 Transposon Insertions by Inverse PCR -- Rapid Amplification of Genomic DNA Sequences Tagged by Insertional Mutagenesis -- Isolation of Large-Terminal Sequences of BAC Inserts Based on Double-Restriction-Enzyme Digestion Followed by Anchored PCR -- A #x201C;Step Down#x201D; PCR-Based Technique for Walking Into and the Subsequent Direct-Sequence Analysis of Flanking Genomic DNA -- Library Construction and Screening -- Use of PCR in Library Screening -- Cloning of Homologous Genes by Gene-Capture PCR -- Rapid and Nonradioactive Screening of Recombinant Libraries by PCR -- Rapid cDNA Cloning by PCR Screening (RC-PCR) -- Generation and PCR Screening of Bacteriophage ? Sublibraries Enriched for Rare Clones (the "Sublibrary Method ") -- PCR-Based Screening for Bacterial Artificial Chromosome Libraries -- A 384-Well Microtiter-Plate-Based Template Preparation and Sequencing Method -- A Microtiter-Plate-Based High Throughput PCR Product Purification Method.
520			_aIn the post-genomic era, PCR has become the method of choice not only for cloning existing genes, but also for generating a wide array of novel genes by mutagenesis and/or recombination within the genes of interest. PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long-distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination and to clone the challenging uncharacterized DNA flanking a known DNA fragment. Powerful applications of PCR in library construction and sublibrary generation and screening are also presented. Authoritative and up-to-date, PCR Cloning Protocols, Second Edition, constitutes a gold-standard collection of the fastest, simplest, and most popular methods for isolating genes from all biological samples and creating novel genes from them by mutagenesis/recombination-essential methods for today's study of functional genomics, gene expression, protein structure-function relationships, protein engineering, and molecular evolution.
988			_aSpringer_Protocols_2002
650		7	_2embne _9159969 _aClonación molecular
776	0	8	_iPrinted edition: _z9781617372810
776	0	8	_iPrinted edition: _z9780896039698
776	0	8	_iPrinted edition: _z9780896039735
776	0	8	_iPrinted edition: _z9781475761467
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592591779 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b01/2024 _dz _eIG _zSI