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008			130217s2013 xxu| o |||| 0|eng d
020			_a9781627033176
024	7		_a10.1007/978-1-62703-317-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH588 .S83 _b2013 EB
245	0	0	_aStem Cells and Aging : _bMethods and Protocols _cedited by Kursad Turksen
250			_a1st edition 2013
264		1	_aTotowa, NJ _bHumana Press _c2013
300			_a1 recurso en línea (XI, 179 páginas) _b30 ilustraciones, 21 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v976
505	0		_aComprehensive Hematopoietic Stem Cell Isolation Methods -- Serial Transplantation of Bone Marrow to Test Self-Renewal Capacity of Hematopoietic Stem Cells In Vivo -- Mouse Hematopoietic Stem Cell Transplantation -- Isolation, Characterization, and Transplantation of Adult Liver Progenitor Cells -- Isolation of Muscle-Derived Stem/Progenitor Cells Based on Adhesion Characteristics to Collagen-Coated Surfaces -- Human Myoblasts from Skeletal Muscle Biopsies: In Vitro Culture Preparations for Morphological and Cytochemical Analyses at Light and Electron Microscopy -- Cardiac Stem Cell Senescence -- Isolation of Mesenchymal Stem Cells from Human Bone and Long-Term Cultivation Under Physiologic Oxygen Conditions -- Methods for Assessing Effects of Wnt/β-Catenin Signaling in Senescence of Mesenchymal Stem Cells -- Intra-Femoral Injection of Human Mesenchymal Stem Cells -- Tracking of Replicative Senescence in Mesenchymal Stem Cells by Colony-Forming Unit Frequency -- Chromatin Immunoprecipitation Coupled by Quantitative Real-Time PCR as a Tool for Analyzing Epigenetic Regulation of Stem Cell Aging -- Quantitative Fluorescence In Situ Hybridization (Q-FISH) on Paraffine Embedded Tissue.
520			_aOver time, it has become clear that changes in stem cells do occur during aging, not only in their number but also in their relationship to their microenvironment and their functionality as reflected in changes to their metabolome.  Stem Cells and Aging: Methods and Protocols brings together chapters from expert contributors with protocols critical for exploring the biology of stem cell aging, all of which is key for understanding these age-related stem cell changes at a basic biology level and at the level of their impacts for regenerative medicine.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.   Concise and easy to use, Stem Cells and Aging: Methods and Protocols serves as an ideal reference to guide investigators toward further valuable answers to the problems of our aging population.
988			_aSpringer_Protocols_2013
650		7	_2embne _9160528 _aCélulas madre
650		7	_2embne _9674670 _aEnvejecimiento _xAspectos genéticos
776	0	8	_iPrinted edition: _z9781627033169
776	0	8	_iPrinted edition: _z9781627033183
776	0	8	_iPrinted edition: _z9781493963034
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-317-6 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b01/2024 _dz _eIG _zSI